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	Aptamer-based electrochemical sensors with aptamer-complementary dna oligonucleotides as probe
	Lu, Ying1,2; Li, Xianchan1,2; Zhang, Limin1,2; Yu, Ping1; Su, Lei1; Mao, Lanqun1
刊名	Analytical chemistry
	2008-03-15
卷号	80期号:6页码:1883-1890
ISSN号	0003-2700
DOI	10.1021/ac7018014
通讯作者	Mao, lanqun(lqmao@iccas.ac.cn)
英文摘要	This study describes a facile and general strategy for the development of aptamer-based electrochemical sensors with a high specificity toward the targets and a ready regeneration feature. very different from the existing strategies for the development of electrochemical aptasensors with the aptamers as the probes, the strategy proposed here is essentially based on the utilization of the aptamer-complementary dna (cdna) oligonucleotides as the probes for electrochemical sensing. in this context, the sequences at both ends of the cdna are tailor-made to be complementary and both the redox moiety (i.e., ferrocene in this study) and thiol group are labeled onto the cdna. the labeled cdna are hybridized with their respective aptamers (i.e., atp- and thrombin-binding aptamers in this study) to form double-stranded dna (dsdna) and the electrochemical aptasensors are prepared by self-assembling the labeled ds-dna onto au electrodes. upon target binding, the aptamers confined onto electrode surface dissociate from their respective cdna oligonucleotides into the solution and the single-stranded cdna could thus tend to form a hairpin structure through the hybridization of the complementary sequences at both its ends. such a conformational. change of the cdna resulting from the target binding-induced dissociation of the aptamers essentially leads to the change in the voltammetric signal of the redox moiety labeled onto the cdna and thus constitutes the mechanism for the electrochemical aptasensors for specific target sensing. the aptasensors demonstrated here with the cdna as the probe are readily regenerated and show good responses toward the targets. this study may offer a new and relatively general approach to electrochemical aptasensors with good analytical properties and potential applications.
WOS关键词	SWITCHING SIGNALING APTAMERS ; SINGLE-STRANDED-DNA ; BLOOD-SERUM ; HAIRPIN ; REAGENTLESS ; RECOGNITION ; APTASENSOR ; MOLECULES ; SEQUENCE ; ATP
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000254017000007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2387371
专题	中国科学院大学
通讯作者	Mao, Lanqun
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Lu, Ying,Li, Xianchan,Zhang, Limin,et al. Aptamer-based electrochemical sensors with aptamer-complementary dna oligonucleotides as probe[J]. Analytical chemistry,2008,80(6):1883-1890.
APA	Lu, Ying,Li, Xianchan,Zhang, Limin,Yu, Ping,Su, Lei,&Mao, Lanqun.(2008).Aptamer-based electrochemical sensors with aptamer-complementary dna oligonucleotides as probe.Analytical chemistry,80(6),1883-1890.
MLA	Lu, Ying,et al."Aptamer-based electrochemical sensors with aptamer-complementary dna oligonucleotides as probe".Analytical chemistry 80.6(2008):1883-1890.

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