Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation

	Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation
	Liang, Yu 2; Cong, Yongzheng 2; Liang, Zhen ; Zhang, Lihua 1; Zhang, Yukui
刊名	electrophoresis
	2009-12-01
卷号	30 期号:23 页码:4034-4039
关键词	Microchip isoelectric focusing Monolithic immobilized pH gradient materials Photopolymerization Proteins
通讯作者	张丽华
产权排序	1;1
英文摘要	monolithic immobilized ph gradient (m-ipg) materials were prepared in microchannles by photoinitiated polymerization of acrylamide, glycidylmethacrylate and bis, followed by the attachment of focused ampholine onto the surface of porous monoliths via epoxide groups. with m-ipg materials as matrix, fitc-labeled ribonuclease b, myoglobin and alpha-casein were well separated by microchip isoelectric focusing (mu cief) without carrier amphocytes (cas) added in the buffer. both chemical and pressure mobilization were applied to drive focused zones for lif detection. our experimental results showed that pressure mobilization was preferable with neglectable band broadening, and good peak shape and high detection sensitivity were obtained. all these results demonstrate that mu cief with m-ipg materials is not only an efficient mode for protein enrichment and separation but also attractive to couple with other ce modes to achieve multi-dimensional separation or ms for further identification, without the interference of mobile cas.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	poly(dimethylsiloxane) microfluidic chip ; temperature-gradient ; gel-electrophoresis ; polyacrylamide-gels ; imaging detection ; integration ; column ; microchannels ; adaptation ; channels
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000272928700006
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/102701]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liang, Yu,Cong, Yongzheng,Liang, Zhen,et al. Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation[J]. electrophoresis,2009,30(23):4034-4039.
APA	Liang, Yu,Cong, Yongzheng,Liang, Zhen,Zhang, Lihua,&Zhang, Yukui.(2009).Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation.electrophoresis,30(23),4034-4039.
MLA	Liang, Yu,et al."Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation".electrophoresis 30.23(2009):4034-4039.