| Jnk regulates cell migration through promotion of tyrosine phosphorylation of paxillin | |
| Huang, Zhi1,2,3; Yan, Da-Peng1,2,3; Ge, Bao-Xue1,2 | |
| 刊名 | Cellular signalling
 |
| 2008-11-01 | |
| 卷号 | 20期号:11页码:2002-2012 |
| 关键词 | Cell migration Jnk Paxillin Fak |
| ISSN号 | 0898-6568 |
| DOI | 10.1016/j.cellsig.2008.07.014 |
| 通讯作者 | Ge, bao-xue(gebaoxue@sibs.ac.cn) |
| 英文摘要 | The adaptor protein paxillin plays an important role in cell migration. although the c-jun amino-terminal kinase (jnk) phosphorylation of paxillin on ser 178 has been found to be critical for cell migration, the precise mechanism by which jnk regulates cell migration is still not very clear. here, the migration of human corneal epithelial (hce) cells was used to determine which signaling pathways are involved in egf-induced paxillin phosphorylation. paxillin was phosphorylated on tyr 31 and tyr 118 after induction of migration by egf in hce cells. specific inhibition of jnk activation by inhibitor sp600125 or overexpression of a dominant-negative jnk mutant not only blocked egf-induced cell migration, but also eliminated tyrosine phosphorylation of paxillin on tyr 31 and tyr 118. hce cells overexpressing paxillin-s178a mutant also exhibited lower mobility, and reduced phosphorylation of tyr 31 and tyr 118. however, paxillin-s178a-inhibited cell migration can be rescued by overexpression of paxillin-y31e/y118e mutant. importantly, inhibition of jnk by sp600125 or overexpression of paxillin-s178a mutant prevented the association of fak with paxillin. taken together, these results suggest that phosphorylation of paxillin on ser 178 by jnk is required for the association of paxillin with fak, and subsequent tyrosine phosphorylation of paxillin. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | EPIDERMAL-GROWTH-FACTOR ; ACTIVATED PROTEIN-KINASE ; CORNEAL EPITHELIAL-CELLS ; FACTOR RECEPTORS ; SIGNALING PATHWAY ; NEURITE EXTENSION ; WOUND REPAIR ; IN-VITRO ; PHOSPHATASE ; DROSOPHILA |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE INC |
| WOS记录号 | WOS:000260637500011 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2385366 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ge, Bao-Xue |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Beijing 100864, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Zhi,Yan, Da-Peng,Ge, Bao-Xue. Jnk regulates cell migration through promotion of tyrosine phosphorylation of paxillin[J]. Cellular signalling,2008,20(11):2002-2012. |
| APA | Huang, Zhi,Yan, Da-Peng,&Ge, Bao-Xue.(2008).Jnk regulates cell migration through promotion of tyrosine phosphorylation of paxillin.Cellular signalling,20(11),2002-2012. |
| MLA | Huang, Zhi,et al."Jnk regulates cell migration through promotion of tyrosine phosphorylation of paxillin".Cellular signalling 20.11(2008):2002-2012. |
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