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	Visualization of cd146 dimerization and its regulation in living cells
	Bu, Pengcheng; Zhuang, Jie; Feng, Jing; Yang, Dongling; Shen, Xun; Yan, Xiyun
刊名	Biochimica et biophysica acta-molecular cell research
	2007-04-01
卷号	1773期号:4页码:513-520
关键词	Cd146 Dimerization Fret Nf-kappa b
ISSN号	0167-4889
DOI	10.1016/j.bbamcr.2007.01.009
通讯作者	Yan, xiyun(yanxy@sun5.ibp.ac.cn)
英文摘要	Our previous study showed that the adhesion molecule cd146 as a biomarker is over-expressed on activated endothelium during angiogenesis, which was induced by tumor conditional medium and inhibited by anti-cd146 monoclonal antibody (mab aa98). however, the cd146 molecular organization on the cells is unknown. here, using immunoprecipitation, we found that the dimerization of cd146 occurs in both normal and tumor cells. however, the dimer/monomer ratio was higher in tumor cells than in normal cells. moreover, we found that cd146 dimerization was up-regulated by turner conditional medium through the nf-kappa b pathway and down-regulated by mab aa98. to further confirm that cd146 dimerization occurs in living cells, we used fluorescence resonance energy transfer (fret) with melanoma mel888 cells co-expressing cfp/yfp-tagged cd146 fusion proteins. by acceptor photobleaching, we observed a strong fret signal produced by these two fluorescence-tagged proteins. the fret efficiency reached 20.1%. our data provide the first evidence that cd146 dimerization occurs in living cells and is regulated within the tumor microenvironment, implying that dimerization of cd146 may be associated with malignancy. (c) 2007 elsevier b.v. all rights reserved.
WOS关键词	ANTI-CD146 MONOCLONAL-ANTIBODY ; ADHESION MOLECULE ; HUMAN-MELANOMA ; TUMOR-GROWTH ; IMMUNOGLOBULIN SUPERFAMILY ; INTERMEDIATE TROPHOBLAST ; INHIBITS ANGIOGENESIS ; ACTIVATION ; EXPRESSION ; PHOSPHORYLATION
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000245784700006
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2383722
专题	中国科学院大学
通讯作者	Yan, Xiyun
作者单位	1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Bu, Pengcheng,Zhuang, Jie,Feng, Jing,et al. Visualization of cd146 dimerization and its regulation in living cells[J]. Biochimica et biophysica acta-molecular cell research,2007,1773(4):513-520.
APA	Bu, Pengcheng,Zhuang, Jie,Feng, Jing,Yang, Dongling,Shen, Xun,&Yan, Xiyun.(2007).Visualization of cd146 dimerization and its regulation in living cells.Biochimica et biophysica acta-molecular cell research,1773(4),513-520.
MLA	Bu, Pengcheng,et al."Visualization of cd146 dimerization and its regulation in living cells".Biochimica et biophysica acta-molecular cell research 1773.4(2007):513-520.

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