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	Avirulence gene and insertion element-based rflp as well as rapd markers reveal high levels of genomic polymorphism in the rice pathogen xanthomonas oryzae pv. oryzae
	Hua, Jun1,2; Zhang, Yan3; Qian, Wei1,4; He, Chaozu1
刊名	Systematic and applied microbiology
	2007-12-01
卷号	30期号:8页码:587-600
关键词	Xanthomonas oryzae pv. oryzae Genetic polymorphism Rflp Avrbs3/ptha gene family Insertion element Rapid
ISSN号	0723-2020
DOI	10.1016/j.syapm.2007.08.003
通讯作者	Qian, wei(qianw@sun.im.ac.cn)
英文摘要	Genetic polymorphism within the genomes of bacterial pathogens determines their evolutionary potential during long-term interaction with their hosts. to investigate the level of genetic variation in xanthomonas oryzae pv. oryzae (xoo), the causative agent of rice bacterial blight disease, three dna marker systems, including (i) restriction fragment length polymorphism (rflp) of the avrbs3/ptha family genes (avrxa27), (ii) rflp of insertion (is) elements and (iii) random amplified polymorphic dna (rapd) markers, were used to detect polymorphism among 32 xoo strains that differed in their virulence patterns. all these strains contained multiple avrxa27 homologs that were variable in copy number and genomic location. rflp of six is elements revealed that these mobile sequences were abundant in xoo genomes, with 150 of the total of 165 discernable markers being variable. thirty-eight decamer primers of rapd amplified a total of 691 bands, with 100% of them being variable. in addition, analysis of molecular variance (amova) of data from rflp analysis of is elements and from rapd analysis showed that most of the genetic variation residues were within xoo populations, rather than between populations. although all three dna marker systems supported that substantial variation was maintained in xoo genomes, mantel tests did not identify significant correlation between the similarity coefficients calculated from them. the results of the present study indicated that xoo genomes contain a high level of genetic polymorphism, which greatly facilitates the evolution of this important pathogen during interaction with its host rice plant. (c) 2007 elsevier grnbh. all rights reserved.
WOS关键词	BACTERIAL-BLIGHT PATHOGEN ; III EFFECTOR ; DIVERSITY ; EVOLUTION ; SEQUENCE ; POPULATION ; RESISTANCE ; PHILIPPINES ; STRAINS ; REGIONS
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	ELSEVIER GMBH, URBAN & FISCHER VERLAG
WOS记录号	WOS:000251655600002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2383543
专题	中国科学院大学
通讯作者	Qian, Wei
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Tsing Hua Univ, Grad Sch Shenzhen, Shenzhen Lab Super Hybrid Rice Res, Shenzhen 518055, Peoples R China 4.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Hua, Jun,Zhang, Yan,Qian, Wei,et al. Avirulence gene and insertion element-based rflp as well as rapd markers reveal high levels of genomic polymorphism in the rice pathogen xanthomonas oryzae pv. oryzae[J]. Systematic and applied microbiology,2007,30(8):587-600.
APA	Hua, Jun,Zhang, Yan,Qian, Wei,&He, Chaozu.(2007).Avirulence gene and insertion element-based rflp as well as rapd markers reveal high levels of genomic polymorphism in the rice pathogen xanthomonas oryzae pv. oryzae.Systematic and applied microbiology,30(8),587-600.
MLA	Hua, Jun,et al."Avirulence gene and insertion element-based rflp as well as rapd markers reveal high levels of genomic polymorphism in the rice pathogen xanthomonas oryzae pv. oryzae".Systematic and applied microbiology 30.8(2007):587-600.

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