Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry

	Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry
	Zhang, Fengxia 2,3; Jia, Zhenhua 1; Gao, Peng 3; Kong, Hongwei 3; Li, Xiang 3; Chen, Jing 3; Yang, Qin 3; Yin, Peiyuan 3; Wang, Jiangshan 3; Lu, Xin 3
刊名	talanta
	2009-08-15
卷号	79 期号:3 页码:836-844
关键词	Atherosclerosis Metabonomics UFLC/MS-IT-TOF Plasma Urine Rats
通讯作者	许国旺
产权排序	1;1
英文摘要	an ultra fast liquid chromatography coupled with it-tof mass spectrometry (uflc/ms-it-tof) metabonomic approach was employed to study the plasma and urine metabolic profiling of atherosclerosis rats. acquired data were subjected to principal component analysis (pca) for differentiating the atherosclerosis and the control groups. potential biomarkers were screened by using s-plot and were identified by the accurate mass and ms(n) fragments information obtained from uflc/ms-it-tof analysis. 12 metabolites in rat plasma and 8 metabolites in urine were identified as potential biomarkers. concentrations of leucine, phenylalanine, tryptophan, acetylcarnitine, butyrylcarnitine, propionylcarnitine and spermine in plasma and 3-o-methyl-dopa, ethyl, n2-acetyl-l-argininate, leucylproline, glucuronate, t6a n(6)-(n-threonylcarbonyl)-adenosine and methyl-hippuric acid in urine decreased in atherosclerosis rats. ursodeoxycholic acid, chenodeoxycholic acid, lpc (06:0), lpc (c18:0) and lpc (c18:1) in plasma and hippuric acid in urine were in higher levels in atherosclerosis rats. the alterated metabolites demonstrated abnormal metabolism of phenylalanine, tryptophan, bile acids and amino acids. this research proved that metabonomics is a promising tool for disease research. (c) 2009 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	multivariate statistical-analysis ; mouse iga nephropathy ; potential biomarkers ; pattern-recognition ; systems biology ; bile-acids ; uplc-ms ; tof-ms ; lysophosphatidylcholine ; identification
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000268346800042
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/102157]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Integrat Tradit & Western Med Res Acad Hebei Prov, Shijiazhuang 050035, Hebei Province, Peoples R China 2.Shenyang Pharmaceut Univ, Sch Pharm, Dept Analyt Chem, Shenyang 110016, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Fengxia,Jia, Zhenhua,Gao, Peng,et al. Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry[J]. talanta,2009,79(3):836-844.
APA	Zhang, Fengxia.,Jia, Zhenhua.,Gao, Peng.,Kong, Hongwei.,Li, Xiang.,...&Xu, Guowang.(2009).Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry.talanta,79(3),836-844.
MLA	Zhang, Fengxia,et al."Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry".talanta 79.3(2009):836-844.