Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device

	Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device
	Li, Chunyu 1,2; Dong, Xiuling 3; Qin, Jianhua 1; Lin, Bingcheng 1
刊名	analytica chimica acta
	2009-04-27
卷号	640 期号:1-2 页码:93-99
关键词	Reciprocating flow DNA hybridization Microfluidics Centrifugal force Capillary action Dengue virus
通讯作者	秦建华 ; 林炳承
产权排序	1;1
英文摘要	we present a compact disk (cd) microfluidic device capable of generating the reciprocating flow of dna samples within the microchannels and demonstrate its application in rapid dna hybridization assay with nanoliter-volume samples. this device consists of a polydimethylsiloxane (pdms) cd slab containing twelve dna hybridization functional units and a glass substrate with immobilized dna probe array. a reciprocating flow is produced with simple rotation-pause operation of the cd device. when spinning the cd device, centrifugal force drives the sample solution to now through the hybridization channel into the temporary collection reservoir. when stopping the rotation of the cd device, capillary action pulls the sample solution to reversely flow back into the hybridization channel because of hydrophilic surface of the hybridization channel. we used dengue virus gene sequence (18mer) as a model to demonstrate that the reciprocating flow of dna samples significantly enhanced the mass transfer rate in the hybridization reaction. reducing the hybridization time to 90 s. moreover, only nanoliter-volume dna samples were required per assay unit. we also compared the performance between the reciprocating-flow hybridization and the flow-through hybridization using the same sample concentrations. a fluorescence intensity was observed to increase up to threefold in the reciprocating-flow hybridization compared to the how-through hybridization with the same hybridization time (90 s) and sample volumes (350 nl). this cd microfluidic device has the potential for automated, rapid and multiple dna-based diagnostics. (c) 2009 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	microarray hybridization ; poly(dimethylsiloxane) ; oligonucleotide ; microchips ; chip ; cd ; mutations ; systems
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000265763500014
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/102113]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Dalian Third Municipal Hosp, Dalian 116033, Peoples R China
推荐引用方式 GB/T 7714	Li, Chunyu,Dong, Xiuling,Qin, Jianhua,et al. Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device[J]. analytica chimica acta,2009,640(1-2):93-99.
APA	Li, Chunyu,Dong, Xiuling,Qin, Jianhua,&Lin, Bingcheng.(2009).Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device.analytica chimica acta,640(1-2),93-99.
MLA	Li, Chunyu,et al."Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device".analytica chimica acta 640.1-2(2009):93-99.