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	Cloning, characterization, and transformation of the phosphoethanolamine n-methyltransferase gene (zmpeamt1) in maize (zea mays l.)
	Wu, Suowei; Yu, Zhanwang; Wang, Fengge; Li, Weihua; Ye, Chunjiang; Li, Jun; Tang, Jihua; Ding, Junqiang; Zhao, Jiuran; Wang, Bin
刊名	Molecular biotechnology
	2007-06-01
卷号	36期号:2页码:102-112
关键词	Maize (zea mays l.) phosphoethanolamine n-methyltransferase promoter Tail-pcr Transgenic plants
ISSN号	1073-6085
DOI	10.1007/s12033-007-0009-1
通讯作者	Wang, bin(bwang@genetics.ac.cn)
英文摘要	N-methylation of phosphoethanolamine, the committing step in choline (cho) biosynthesis in plants, is catalyzed by s-adenosyl-l-methionine: phosphoethanolamine n-methyltransferase (peamt, ec 2.1.1.103). herein we report the cloning and characterization of the novel maize phosphoethanolarnine n-methyltransferase gene (zmpeamt1) using a combination of bioinformatics and a pcr-based allele mining strategy. the cdna sequence of zmpeamt] gene is 1,806 bp in length and translates a 495 amino acids peptide. the upstream promoter sequence of zmpeamt1 were obtained by tail-pcr, and contained four kinds of putative cis-acting regulatory elements, including stress-responsive elements, phytohormone-responsive elements, pollen developmental special activation elements, and light-induced signal transduction elements, as well as several other structural features in common with the promoter of rice and arabidopsis homologies. rt-pcr analysis showed that expression of zmpeamt1 was induced by salt stress and suppressed by high temperature. over-expression of zmpeamt1 enhanced the salt tolerance, root length, and silique number in transgenic arabidopsis. these data indicated that zmpeamt1 maybe involved in maize root development and stress resistance, and maybe having a potential application in maize genetic engineering.
WOS关键词	TRANSGENIC TOBACCO PLANTS ; CIS-REGULATORY ELEMENTS ; ARABIDOPSIS-THALIANA ; GLYCINE BETAINE ; TRANSCRIPTION FACTORS ; PHOSPHATIDIC-ACID ; CHOLINE OXIDASE ; CODA GENE ; GCC-BOX ; EXPRESSION
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	HUMANA PRESS INC
WOS记录号	WOS:000247271300003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2382904
专题	中国科学院大学
通讯作者	Wang, Bin
作者单位	1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Maize Res Ctr, Beijing 100089, Peoples R China 4.Henan Agr Univ, Coll Agron, Zhengzhou 450002, Peoples R China
推荐引用方式 GB/T 7714	Wu, Suowei,Yu, Zhanwang,Wang, Fengge,et al. Cloning, characterization, and transformation of the phosphoethanolamine n-methyltransferase gene (zmpeamt1) in maize (zea mays l.)[J]. Molecular biotechnology,2007,36(2):102-112.
APA	Wu, Suowei.,Yu, Zhanwang.,Wang, Fengge.,Li, Weihua.,Ye, Chunjiang.,...&Wang, Bin.(2007).Cloning, characterization, and transformation of the phosphoethanolamine n-methyltransferase gene (zmpeamt1) in maize (zea mays l.).Molecular biotechnology,36(2),102-112.
MLA	Wu, Suowei,et al."Cloning, characterization, and transformation of the phosphoethanolamine n-methyltransferase gene (zmpeamt1) in maize (zea mays l.)".Molecular biotechnology 36.2(2007):102-112.

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