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	Vascularization and cellularization of collagen scaffolds incorporated with two different collagen-targeting human basic fibroblast growth factors
	Zhao, Wenxue; Chen, Bing; Li, Xiaodong; Lin, Hang; Sun, Wenjie; Zhao, Yanhong; Wang, Bin; Zhao, Yannan; Han, Qianqian; Dai, Jianwu
刊名	Journal of biomedical materials research part a
	2007-09-01
卷号	82a期号:3页码:630-636
关键词	Bfgf Collagen-binding domain Targeting repair Vascularization Cellullarization
ISSN号	1549-3296
DOI	10.1002/bjm.a.31179
通讯作者	Dai, jianwu(jwdai@genetics.ac.cn)
英文摘要	To develop a collagen-based wound targeting repair system, we introduced two collagen-binding domains (cbds) into the human basic fibroblast growth factor (bfgf). three expression vectors were constructed: the first one (named v-bfgf) contained bfgf and the cbd wrepsf-cals derived from von willeband's factor (vwf); the second (named c-bfgf) contained bfgf and the cbd tkktlrt derived from collagenase; the third (named bfgf) was bfgf as a control. the recombinant proteins of v-bfgf and c-bfgf were demonstrated to retain both growth factor activity and collagen-binding activity. we found that c-bfgf possessed higher collagen-binding ability than v-bfgf. the targeted repair systems consisting of collagen scaffolds and cbd-bfgfs were assembled in vitro and then implanted subcutaneously. results showed that c-bfgf promoted vascularization at the implanted sites more effectively than v-bfgf. histological analysis showed more cells migrated into collagen scaffolds incorporated with c-bfgf than those with v-bfgf. these data suggested that the higher collagen-binding ability the cbd-bfgf possessed, the more significant vascularization, and cellularization were observed. in summary, cbd-bfgf/collagen system could be used as a targeted repair system with beneficial effects of the restriction of bfgf diffusion, the prolonging of bfgf activity, and the targeted promotion of vascularization and cellularization. (c) 2007 wiley periodicals, inc.
WOS关键词	CONTROLLED-RELEASE ; BINDING ; HYDROGELS ; HEPARIN ; FUSION ; PROLIFERATION ; DOMAIN
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
语种	英语
出版者	WILEY-LISS
WOS记录号	WOS:000248693100012
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2382062
专题	中国科学院大学
通讯作者	Dai, Jianwu
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Cent Hosp Aviat Ind China, Dept Plast Surg, Beijing 100012, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Wenxue,Chen, Bing,Li, Xiaodong,et al. Vascularization and cellularization of collagen scaffolds incorporated with two different collagen-targeting human basic fibroblast growth factors[J]. Journal of biomedical materials research part a,2007,82a(3):630-636.
APA	Zhao, Wenxue.,Chen, Bing.,Li, Xiaodong.,Lin, Hang.,Sun, Wenjie.,...&Dai, Jianwu.(2007).Vascularization and cellularization of collagen scaffolds incorporated with two different collagen-targeting human basic fibroblast growth factors.Journal of biomedical materials research part a,82a(3),630-636.
MLA	Zhao, Wenxue,et al."Vascularization and cellularization of collagen scaffolds incorporated with two different collagen-targeting human basic fibroblast growth factors".Journal of biomedical materials research part a 82a.3(2007):630-636.

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