Graphitized macroporous carbon microarray with hierarchical mesopores as host for the fabrication of electrochemical biosensor

	Graphitized macroporous carbon microarray with hierarchical mesopores as host for the fabrication of electrochemical biosensor
	Lu, Xianbo 1; Xiao, Yi 2; Lei, Zhibin 2; Chen, Jiping 1
刊名	biosensors & bioelectronics
	2009-09-15
卷号	25 期号:1 页码:244-247
关键词	Biosensor Graphitized ordered macroporous carbon Direct electron transfer Hemoglobin Hydrogen peroxide
通讯作者	陈吉平
产权排序	1;1
英文摘要	a novel graphitized ordered macroporous carbon (gmc, pore size similar to 380 nm) with hierarchical mesopores (2-30 nm) and high graphitization degree was prepared by nickel-catalyzed graphitization of polystyrene arrays. the obtained gmc possessed high specific surface area, large pore volume, and good electrical conductivity, which was explored for the enzyme entrapment and biosensor fabrication by a facile method. with advantages of novel nanostructure and good electrical conductivity, direct electrochemistry of hemoglobin (a model protein) was observed on the gmc-based biocomposite with a formal potential of -0.36v (vs. ag/agcl) and an apparent heterogeneous electron transfer rate constant (k(s)) of 1.2 s(-1) in ph 7.0 buffer. comparative studies revealed that gmc offered significant advantages over carbon nanotubes (cnts) in facilitating direct electron transfer of entrapped hb. the fabricated biosensor exhibited good sensitivity (101.6 ma cm(-2) m-1) and reproducibility, wide linear range (1-267 mu m), low detection limit (0.1 mu m), and good long-term stability for h2o2 detection. gmc proved to be a promising matrix for enzyme entrapment and biosensor fabrication, and may find wide potential applications in biomedical detection and environmental analyses. (c) 2009 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biophysics ; biotechnology & applied microbiology ; chemistry, analytical ; electrochemistry ; nanoscience & nanotechnology
研究领域[WOS]	biophysics ; biotechnology & applied microbiology ; chemistry ; electrochemistry ; science & technology - other topics
关键词[WOS]	direct electron-transfer ; peroxidase-activity ; glucose biosensor ; construction ; functionalization ; proteins ; systems ; layer
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000270538400040
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/101793]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Liaoning Normal Univ, Fac Chem & Chem Engn, Inst Chem Functionalized Mat, Dalian 116029, Peoples R China
推荐引用方式 GB/T 7714	Lu, Xianbo,Xiao, Yi,Lei, Zhibin,et al. Graphitized macroporous carbon microarray with hierarchical mesopores as host for the fabrication of electrochemical biosensor[J]. biosensors & bioelectronics,2009,25(1):244-247.
APA	Lu, Xianbo,Xiao, Yi,Lei, Zhibin,&Chen, Jiping.(2009).Graphitized macroporous carbon microarray with hierarchical mesopores as host for the fabrication of electrochemical biosensor.biosensors & bioelectronics,25(1),244-247.
MLA	Lu, Xianbo,et al."Graphitized macroporous carbon microarray with hierarchical mesopores as host for the fabrication of electrochemical biosensor".biosensors & bioelectronics 25.1(2009):244-247.