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	Effects of ph and nitrogen forms on expression profiles of genes involved in iron homeostasis in tomato
	Zhao, Ting; Ling, Hong-Qing
刊名	Plant cell and environment
	2007-04-01
卷号	30期号:4页码:518-527
关键词	Ammonium Gene expression Nitrate
ISSN号	0140-7791
DOI	10.1111/j.1365-3040.2007.01638.x
通讯作者	Ling, hong-qing(hqling@genetics.ac.cn)
英文摘要	In this work, we investigated the effects of ph and nitrogen forms on iron homeostasis and the expression profiles of genes involved in iron uptake and metabolism using tomato cultivar t3238 and its iron-inefficient mutant t3238fer. we showed that high external ph led to increased expression of four iron uptake genes (leirt1, leirt2, lefro1, lenramp1) regardless of the nitrogen sources. interestingly, the transcript level of fer was decreased at high ph and increased at low ph. in iron-inefficient mutant t3238fer, the expression of lefro1, leirt1 and lenramp1 was much less than wild type under the culture conditions with high ph and on the non-buffered agar medium with no3- as the sole n source, demonstrating that fer protein is required for the increased expression of lefro1, leirt1 and lenramp1 under culture conditions with high ph. considering the paradoxical expression patterns of fer to lefro1, leirt1 and lenramp1 in t3238, we speculate that fer is essential, but is not the limited factor for the transcriptional regulation of the three iron uptake genes. in conclusion, the alteration of rhizosphere ph by assimilating no3- or nh4+ influenced fe availability and consequently affected iron homeostasis in tomato. the enhanced expression of lefro1, leirt1 and lenramp1 under the culture condition with high ph or on agar media with no3- as the sole n source might be a consequence of reduced iron availability in the solution or agar medium at high ph.
WOS关键词	FERRIC-CHELATE REDUCTASE ; FE DEFICIENCY CHLOROSIS ; HELIANTHUS-ANNUUS L. ; MAP-BASED CLONING ; SUNFLOWER LEAVES ; ARABIDOPSIS-THALIANA ; METAL TRANSPORTER ; APOPLASTIC PH ; PLANTS ; LEAF
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000244419700013
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380633
专题	中国科学院大学
通讯作者	Ling, Hong-Qing
作者单位	1.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Ting,Ling, Hong-Qing. Effects of ph and nitrogen forms on expression profiles of genes involved in iron homeostasis in tomato[J]. Plant cell and environment,2007,30(4):518-527.
APA	Zhao, Ting,&Ling, Hong-Qing.(2007).Effects of ph and nitrogen forms on expression profiles of genes involved in iron homeostasis in tomato.Plant cell and environment,30(4),518-527.
MLA	Zhao, Ting,et al."Effects of ph and nitrogen forms on expression profiles of genes involved in iron homeostasis in tomato".Plant cell and environment 30.4(2007):518-527.

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