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	Trehalose as a good candidate for enriching full-length cdnas in cdna library construction
	Chen, Lei; Cao, Lixue; Zhou, Longhai; Jing, Yudong; Chen, Zuozhou; Deng, Cheng; Shen, Yu; Chen, Liangbiao
刊名	Journal of biotechnology
	2007-01-10
卷号	127期号:3页码:402-407
关键词	Stratascript rt Reverse transcript Full-length cdna library
ISSN号	0168-1656
DOI	10.1016/j.jbiotec.2006.07.033
通讯作者	Chen, liangbiao(lbchen@genetics.ac.cn)
英文摘要	It has been reported that the disaccharide trehalose is capable of increasing the thermostability and thermoactivity of reverse transcriptase, and therefore improving the length of cdna synthesis. however, no test has been done on how the disaccharide trehalose performs in the context of the entire cdna synthesis processes, or whether it can seamlessly integrate into the commercially available cdna synthesis kit. in this report, we optimized a protocol to incorporate trehalose in the stratagene's cdna library construction kit in order to demonstrate great improvement in cdna's length (average length of 1.8 kb in the trehalose group versus 1.0 kb in the control). sequence analysis of the cdna clones showed that the addition of trehalose did not increase the error rate of the rt products but greatly increase the quantity of full-length in cdna library. (c) 2006 elsevier b.v. all rights reserved.
WOS关键词	PCR ; STABILIZATION ; AMPLIFICATION ; EFFICIENCY ; ENZYMES ; CLONES ; PROBES ; YEAST ; RNA
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000243697400008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380417
专题	中国科学院大学
通讯作者	Chen, Liangbiao
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Chinese Agr Univ, Coll Anim Med, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Chen, Lei,Cao, Lixue,Zhou, Longhai,et al. Trehalose as a good candidate for enriching full-length cdnas in cdna library construction[J]. Journal of biotechnology,2007,127(3):402-407.
APA	Chen, Lei.,Cao, Lixue.,Zhou, Longhai.,Jing, Yudong.,Chen, Zuozhou.,...&Chen, Liangbiao.(2007).Trehalose as a good candidate for enriching full-length cdnas in cdna library construction.Journal of biotechnology,127(3),402-407.
MLA	Chen, Lei,et al."Trehalose as a good candidate for enriching full-length cdnas in cdna library construction".Journal of biotechnology 127.3(2007):402-407.

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