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	Nitric oxide controls nuclear export of ape1/ref-1 through s-nitrosation of cysteines 93 and 310
	Qu, Jing; Liu, Guang-Hui; Huang, Bo; Chen, Chang
刊名	Nucleic acids research
	2007-04-01
卷号	35期号:8页码:2522-2532
ISSN号	0305-1048
DOI	10.1093/nar/gkl1163
通讯作者	Chen, chang(changchen@moon.ibp.ac.cn)
英文摘要	Apurinic/apyrimidinic endonuclease 1/redox effector factor-1 (ape1/ref-1, abbreviated as ape1) is a molecule with dual functions in dna repair and redox regulation of transcription factors. accumulated work has shown that the biological activities of ape1 are sensitive to oxidative stress; however, whether ape1 functions can be regulated by nitrosative stress remains unknown. in this investigation, we found that s-nitrosoglutathion (gsno), a nitric oxide donor and also an s-nitrosating agent, effectively stimulated nuclear export of ape1 in a crm1-independent manner. this nuclear-cytoplasmic translocation was dependent on s-nitrosation modification of ape1, as simultaneous mutation of s-nitrosation target sites cys93 and cys310 completely abrogated the cytoplasmic redistribution. the translocation process was reversal and specific, as it could be reversed by reductive reagents, but could not be mimicked by h2o2. in structure, the region aa. 64-80 and the beta-strand aa.311-316 in proximity to cys93 and cys310 were important for gsno-induced ape1 relocalization. in addition, a defect of importin-mediated nuclear import pathway was found in the no-insulted cells, and p50 and hdac2 were identified as ape1 nuclear export inhibitory proteins. together, this study may provide a novel molecular mechanism, which links nitrosative stress to ape1-associated physiological and pathological processes.
WOS关键词	DNA-REPAIR ENZYME ; HUMAN APURINIC/APYRIMIDINIC ENDONUCLEASE ; TRANSCRIPTIONAL ACTIVITY ; APOPTOTIC FUNCTIONS ; GENE-TRANSCRIPTION ; PROTEIN APE/REF-1 ; OXIDATIVE STRESS ; REDOX REGULATION ; MAMMALIAN-CELLS ; ABASIC SITES
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000247239600007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380332
专题	中国科学院大学
通讯作者	Chen, Chang
作者单位	1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Qu, Jing,Liu, Guang-Hui,Huang, Bo,et al. Nitric oxide controls nuclear export of ape1/ref-1 through s-nitrosation of cysteines 93 and 310[J]. Nucleic acids research,2007,35(8):2522-2532.
APA	Qu, Jing,Liu, Guang-Hui,Huang, Bo,&Chen, Chang.(2007).Nitric oxide controls nuclear export of ape1/ref-1 through s-nitrosation of cysteines 93 and 310.Nucleic acids research,35(8),2522-2532.
MLA	Qu, Jing,et al."Nitric oxide controls nuclear export of ape1/ref-1 through s-nitrosation of cysteines 93 and 310".Nucleic acids research 35.8(2007):2522-2532.

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