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	The n-terminal double-stranded rna binding domains of arabidopsis hyponastic leaves1 are sufficient for pre-microrna processing
	Wu, Feijie; Yu, Lin; Cao, Wenguang; Mao, Yanfei; Liu, Zhongyuan; He, Yuke
刊名	Plant cell
	2007-03-01
卷号	19期号:3页码:914-925
ISSN号	1040-4651
DOI	10.1105/tpc.106.048637
通讯作者	He, yuke(ykhe@sibs.ac.cn)
英文摘要	Arabidopsis thaliana hyponastic leaves1 (hyl1) is a microrna (mirna) biogenesis protein that contains two n-terminal double-stranded rna binding domains (dsrbds), a putative nuclear localization site (nls), and a putative protein-protein interaction domain. the interaction of hyl1 with dicer-like1 is important for the efficient and precise processing of mirna primary transcripts in plant mirna biogenesis. to define the roles of the various domains of hyl1 in mirna processing and the mirna-directed phenotype, we transferred a series of hyl1 deletion constructs into hyl1 null mutants. the n-terminal region containing dsrbd1 and dsrbd2 completely rescued the mutant phenotype of hyl1, triggering the accumulation of mir166 and mir160 and resulting in reduced mrna levels of the targeted genes. in vivo biochemical analysis of the hyl1-containing complexes from the transgenic plants revealed that the n-terminal dsrbds of hyl1 were sufficient for processing mirna precursors and the generation of mature mirna. transient and stable expression analysis demonstrated that the putative nls domain was indeed the nuclear localization signal, whereas the n-terminal region containing the dsrbds was not restricted to the nucleus. we suggest that the n-terminal dsrbds fulfill the function of the whole hyl1 and thus play an essential role in mirna processing and mirna-directed silencing of targeted genes.
WOS关键词	PLANT DEVELOPMENT ; NUCLEAR EXPORT ; PROTEIN HYL1 ; GENE ; DSRNA ; DICER ; MIRNA ; THALIANA ; SERRATE ; ACCUMULATION
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000246132600017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380204
专题	中国科学院大学
通讯作者	He, Yuke
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Wu, Feijie,Yu, Lin,Cao, Wenguang,et al. The n-terminal double-stranded rna binding domains of arabidopsis hyponastic leaves1 are sufficient for pre-microrna processing[J]. Plant cell,2007,19(3):914-925.
APA	Wu, Feijie,Yu, Lin,Cao, Wenguang,Mao, Yanfei,Liu, Zhongyuan,&He, Yuke.(2007).The n-terminal double-stranded rna binding domains of arabidopsis hyponastic leaves1 are sufficient for pre-microrna processing.Plant cell,19(3),914-925.
MLA	Wu, Feijie,et al."The n-terminal double-stranded rna binding domains of arabidopsis hyponastic leaves1 are sufficient for pre-microrna processing".Plant cell 19.3(2007):914-925.

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