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	Expression analysis of the glutamine synthetase and glutamate synthase gene families in young rice (oryza sativa) seedlings
	Zhao, XQ; Shi, WM
刊名	Plant science
	2006-04-01
卷号	170期号:4页码:748-754
关键词	Gene expression Glutamate synthase Glutamine synthetase Real-time pcr Rice seedlings
ISSN号	0168-9452
DOI	10.1016/j.plantsci.2005.11.006
通讯作者	Shi, wm()
英文摘要	Glutamine synthetase (gs) and glutamate synthase (gogat) serve for primary assimilation of n in higher plants. when nh4+ is the major inorganic n source, rice plants must assimilate nh4+ quickly in roots through the gs/gogat cycle to ameliorate the toxic effect of excess nh4+. the sequence of the rice genome is almost complete, facilitating the identification of the gs and gogat gene families in this species. thus, we investigated the different members of gs and gogat genes, and analyzed the pattern of expression of each gene in young rice seedlings by quantitative real-time pcr, revealing a distinct expression pattern for these genes. osgln1;1 and osgln1;2 mainly functions in roots, and osgln2 and osglu1 are preferentially expressed in leaves. however, transcriptions of osgln1;1, osgln1;2, osgln2 and osglu1 in leaves are all increased by increased n level while those in roots not influenced or even decreased. osglt1 and osglt2 are expressed primarily in roots when n is limiting, but in leaves when non-limiting. transcription of osglt1 is decreased, but osglt2 is increased by increased n level in roots and leaves. when rice roots are exposed to no3- and nh4+ for 2 h after n-starvation, transcriptions of osgln1;1, osglt1, osglt2 and osglu1 are all repressed by no3- and nh4+. osgln1;2 expression shows significant up-regulation by nf14' and down-regulation by no3- while osgln2 down-regulation by nh4+ and up-regulation by no3-. (c) 2005 elsevier ireland ltd. all rights reserved.
WOS关键词	AMMONIUM ASSIMILATION ; NITROGEN ASSIMILATION ; ACID-METABOLISM ; PLANTS ; NITRATE ; ROOTS ; PROTEIN ; BARLEY ; ARABIDOPSIS ; PATHWAY
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000236078900008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379240
专题	中国科学院大学
通讯作者	Shi, WM
作者单位	1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhao, XQ,Shi, WM. Expression analysis of the glutamine synthetase and glutamate synthase gene families in young rice (oryza sativa) seedlings[J]. Plant science,2006,170(4):748-754.
APA	Zhao, XQ,&Shi, WM.(2006).Expression analysis of the glutamine synthetase and glutamate synthase gene families in young rice (oryza sativa) seedlings.Plant science,170(4),748-754.
MLA	Zhao, XQ,et al."Expression analysis of the glutamine synthetase and glutamate synthase gene families in young rice (oryza sativa) seedlings".Plant science 170.4(2006):748-754.

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