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	An improved method for isolation of photosystem ii from marine alga porphyra yezoensis udea
	Gao, ZQ; Wang, GC; Tseng, CK
刊名	Indian journal of biochemistry & biophysics
	2005-02-01
卷号	42期号:1页码:41-47
关键词	Porphyra yezoensis udea Thylakoid membranes Psii Ultracentrifugation Differential speeds centrifugation Sds-page Absorption spectra Fluorescence spectra
ISSN号	0301-1208
通讯作者	Wang, gc()
英文摘要	An improved method for isolation and characterization of photosystem (ps)ii particles from thylakoid membranes of gametophytes. of a marine alga porphyra yezoensis udea is reported. thylakoid membranes were isolated using ultracentrifugation and differential speeds centrifugation and were further purified by the first sucrose density gradient centrifugation (sdgc). psii particles with high 2, 6-dichloroindophenol (dcip) photo-reduction activity were isolated by the second sdgc from the thylakoid membranes. absorption and fluorescence spectra of the thylakoid membranes and psii particles were recorded and their polypeptides composition was studied. thylakoid membranes obtained by the above two methods showed similar spectral properties and polypeptides composition. psii particles, in addition to common extrinsic proteins found in psii of other plants, contained cyt c-550, a 20 kda protein, along with two new proteins (14 kda and 16 kda).
WOS关键词	CHLOROPHYLL-PROTEIN COMPLEXES ; RED ALGA ; EXTRINSIC PROTEINS ; CORE COMPLEX ; FUNCTIONAL-PROPERTIES ; THYLAKOID MEMBRANES ; CYANIDIUM-CALDARIUM ; CRYSTAL-STRUCTURE ; RESOLUTION ; BINDING
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	NATL INST SCIENCE COMMUNICATION
WOS记录号	WOS:000227505100006
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378308
专题	中国科学院大学
通讯作者	Wang, GC
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Gao, ZQ,Wang, GC,Tseng, CK. An improved method for isolation of photosystem ii from marine alga porphyra yezoensis udea[J]. Indian journal of biochemistry & biophysics,2005,42(1):41-47.
APA	Gao, ZQ,Wang, GC,&Tseng, CK.(2005).An improved method for isolation of photosystem ii from marine alga porphyra yezoensis udea.Indian journal of biochemistry & biophysics,42(1),41-47.
MLA	Gao, ZQ,et al."An improved method for isolation of photosystem ii from marine alga porphyra yezoensis udea".Indian journal of biochemistry & biophysics 42.1(2005):41-47.

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