QQ客服 官方微博 反馈留言

	Expansion of nk cells from pbmcs using immobilized 4-1bbl and interleukin-21
	Li, Xiaomei1,3; He, Chenhui1; Liu, Changzhen1; Ma, Juan1; Ma, Pan1,3; Cui, Honglian1; Tao, Hua1; Gao, Bin1,2
刊名	International journal of oncology
	2015-07-01
卷号	47期号:1页码:335-342
ISSN号	1019-6439
DOI	10.3892/ijo.2015.3005
通讯作者	Gao, bin(bgao2004@gmail.com)
英文摘要	Adoptive transfer of nk cells has been widely applied clinically for cancer immunotherapy. however, the difficulties to obtain a large number of activated nk cells impede the successful application of such therapy. in the present study, we implemented a novel method involving the use of immobilized human 4-1 bbl and interleukin-21 to amplify nk cells from the peripheral blood mononuclear cells (pbmcs) of healthy donors. following stimulation for 21 days, we achieved considerable expansion of nk cells with high purity and strong cytotoxicity. this is the first time solid phase cytokines were used to augment nk cells, and this method has the advantage of no need to introduce feeder cells, without prior purification of nk cells and it effectively stimulated and expanded nk cells. the strategy of cell proliferation and activation could lead to a safer and more effective application of nk cells clinically.
WOS关键词	NATURAL-KILLER-CELLS ; EX-VIVO EXPANSION ; LARGE-SCALE ; COSTIMULATORY MOLECULE ; CLINICAL-APPLICATIONS ; EXTRACELLULAR DOMAIN ; INHIBITORY SIGNALS ; ANTITUMOR-ACTIVITY ; IL-21 RECEPTOR ; T-CELLS
WOS研究方向	Oncology
WOS类目	Oncology
语种	英语
出版者	SPANDIDOS PUBL LTD
WOS记录号	WOS:000356468100037
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376494
专题	中国科学院大学
通讯作者	Gao, Bin
作者单位	1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol CASPMI, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Microbiol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaomei,He, Chenhui,Liu, Changzhen,et al. Expansion of nk cells from pbmcs using immobilized 4-1bbl and interleukin-21[J]. International journal of oncology,2015,47(1):335-342.
APA	Li, Xiaomei.,He, Chenhui.,Liu, Changzhen.,Ma, Juan.,Ma, Pan.,...&Gao, Bin.(2015).Expansion of nk cells from pbmcs using immobilized 4-1bbl and interleukin-21.International journal of oncology,47(1),335-342.
MLA	Li, Xiaomei,et al."Expansion of nk cells from pbmcs using immobilized 4-1bbl and interleukin-21".International journal of oncology 47.1(2015):335-342.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。