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	Functional diversity of anti-lipopolysaccharide factor isoforms in shrimp and their characters related to antiviral activity
	Li, Shihao1,2; Guo, Shuyue1,3; Li, Fuhua1,2; Xiang, Jianhai1
刊名	Marine drugs
	2015-05-01
卷号	13期号:5页码:2602-2616
关键词	Anti-lipopolysaccharide factor Lps-binding domain Antibacterial Antiviral Lysine residue
ISSN号	1660-3397
DOI	10.3390/md13052602
通讯作者	Li, fuhua(fhli@qdio.ac.cn)
英文摘要	Anti-lipopolysaccharide factor (alf) is a small protein with broad-spectrum antimicrobial activity, which has potential application in the disease control. previously, we isolated seven alf isoforms from the chinese shrimp fenneropenaeuschinensis. in the present study, their distributions in tissues of shrimp were analyzed and the data showed that different isoforms had different expression profiles, which suggested that they might have different functions. then, the functions of different isoforms were studied by analyzing the antibacterial and antiviral activities of the functional domain of alfs, the lps-binding domain (lbd), which were synthesized by chemical methods. different alfs showed distinct antibacterial and antiviral activities, which were consistent with their diverse tissue distribution patterns. sequence analysis on the lbd domain of different isoforms revealed that an identical lysine residue site was specifically conserved in peptides with anti-wssv activity. in order to confirm whether this lysine residue is critical to the antiviral activity of the peptide, new peptides were synthesized by changing residues at this site. changing the lysine residue at the specific site to other amino acid residue, the antiviral activity of the peptide apparently decreased. while replacing other residue with a lysine residue at this site in lbd peptide without anti-wssv activity, the peptide will obtain the antiviral activity to wssv. these results not only showed us a comprehensive understanding on the function of alfs from f. chinensis, but also provided clues for the development of alfs as potential therapeutic drugs to wssv.
WOS关键词	BLACK TIGER SHRIMP ; CRAB PORTUNUS-TRITUBERCULATUS ; SPOT SYNDROME VIRUS ; LPS-BINDING DOMAIN ; PENAEUS-MONODON ; ANTIMICROBIAL ACTIVITY ; MOLECULAR-CLONING ; FENNEROPENAEUS-CHINENSIS ; LYMPHOID ORGAN ; FACTOR ALF
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000356241100003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376481
专题	中国科学院大学
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Shihao,Guo, Shuyue,Li, Fuhua,et al. Functional diversity of anti-lipopolysaccharide factor isoforms in shrimp and their characters related to antiviral activity[J]. Marine drugs,2015,13(5):2602-2616.
APA	Li, Shihao,Guo, Shuyue,Li, Fuhua,&Xiang, Jianhai.(2015).Functional diversity of anti-lipopolysaccharide factor isoforms in shrimp and their characters related to antiviral activity.Marine drugs,13(5),2602-2616.
MLA	Li, Shihao,et al."Functional diversity of anti-lipopolysaccharide factor isoforms in shrimp and their characters related to antiviral activity".Marine drugs 13.5(2015):2602-2616.

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