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	Hyphopodium-specific vdnoxb/vdpls1-dependent ros-ca2+ signaling is required for plant infection by verticillium dahliae
	Zhao, Yun-Long1,2,3; Zhou, Ting-Ting1,2,3; Guo, Hui-Shan1,2
刊名	Plos pathogens
	2016-07-01
卷号	12期号:7页码:23
ISSN号	1553-7366
DOI	10.1371/journal.ppat.1005793
通讯作者	Guo, hui-shan(guohs@im.ac.cn)
英文摘要	Verticillium dahliae is a phytopathogenic fungus obligate in root infection. a few hyphopodia differentiate from large numbers of hyphae after conidia germination on the root surface for further infection. however, the molecular features and role of hyphopodia in the pathogenicity of v. dahliae remain elusive. in this study, we found that the vdpls1, a tetraspanin, and the vdnoxb, a catalytic subunit of membrane-bound nadph oxidases for reactive oxygen species (ros) production, were specifically expressed in hyphopodia. vdpls1 and vdnoxb highly co-localize with the plasma membrane at the base of hyphopodia, where ros and penetration pegs are generated. mutant strains, vd delta noxb and vd delta pls1, in which vdpls1 and vdnoxb were deleted, respectively, developed defective hyphpodia incapable of producing ros and penetration pegs. defective plasma membrane localization of vdnoxb in vd delta pls1 demonstrates that vdpls1 functions as an adaptor protein for the recruitment and activation of the vdnoxb. furthermore, in vd delta noxb and vd delta pls1, tip-high ca2+ accumulation was impaired in hyphopodia, but not in vegetative hyphal tips. moreover, nuclear targeting of vdcrz1 and activation of calcineurin-crz1 signaling upon hyphopodium induction in wild-type v. dahliae was impaired in both knockout mutants, indicating that vdpls1/vdnoxb-dependent ros was specifically required for tip-high ca2+ elevation in hyphopodia to activate the transcription factor vdcrz1 in the regulation of penetration peg formation. together with the loss of virulence of vd delta noxb and vd delta pls1, which are unable to initiate colonization in cotton plants, our data demonstrate that vdnoxb/vdpls1-mediated ros production activates vdcrz1 signaling through ca2+ elevation in hyphopodia, infectious structures of v. dahliae, to regulate penetration peg formation during the initial colonization of cotton roots.
WOS关键词	NADPH OXIDASE COMPLEX ; RICE BLAST FUNGUS ; APPRESSORIUM-MEDIATED PENETRATION ; HUMAN BLOOD PHAGOCYTES ; REACTIVE OXYGEN ; BOTRYTIS-CINEREA ; ASCOSPORE GERMINATION ; ASPERGILLUS-NIDULANS ; ARABIDOPSIS-THALIANA ; MAGNAPORTHE-GRISEA
WOS研究方向	Microbiology ; Parasitology ; Virology
WOS类目	Microbiology ; Parasitology ; Virology
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000383366400051
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376062
专题	中国科学院大学
通讯作者	Guo, Hui-Shan
作者单位	1.Inst Microbiol, State Key Lab Plant Genom, Beijing, Peoples R China 2.Inst Microbiol, Natl Ctr Plant Gene Res Beijing, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Yun-Long,Zhou, Ting-Ting,Guo, Hui-Shan. Hyphopodium-specific vdnoxb/vdpls1-dependent ros-ca2+ signaling is required for plant infection by verticillium dahliae[J]. Plos pathogens,2016,12(7):23.
APA	Zhao, Yun-Long,Zhou, Ting-Ting,&Guo, Hui-Shan.(2016).Hyphopodium-specific vdnoxb/vdpls1-dependent ros-ca2+ signaling is required for plant infection by verticillium dahliae.Plos pathogens,12(7),23.
MLA	Zhao, Yun-Long,et al."Hyphopodium-specific vdnoxb/vdpls1-dependent ros-ca2+ signaling is required for plant infection by verticillium dahliae".Plos pathogens 12.7(2016):23.

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