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	Unpolarized release of vaccinia virus and hiv antigen by colchicine treatment enhances intranasal hiv antigen expression and mucosal humoral responses
	Zhang, Yan1; Yang, Jingyi1; Bao, Rong1; Chen, Yaoqing2,3; Zhou, Dihan1; He, Benxia1; Zhong, Maohua1; Li, Yaoming1; Liu, Fang1; Li, Qiaoli1
刊名	Plos one
	2011-09-15
卷号	6期号:9页码:13
ISSN号	1932-6203
DOI	10.1371/journal.pone.0024296
通讯作者	Zhang, yan()
英文摘要	The induction of a strong mucosal immune response is essential to building successful hiv vaccines. highly attenuated recombinant hiv vaccinia virus can be administered mucosally, but even high doses of immunization have been found unable to induce strong mucosal antibody responses. in order to solve this problem, we studied the interactions of recombinant hiv vaccinia virus tiantan strain (rvtt-gagpol) in mucosal epithelial cells (specifically caco-2 cell layers) and in balb/c mice. we evaluated the impact of this virus on hiv antigen delivery and specific immune responses. the results demonstrated that rvtt-gagpol was able to infect caco-2 cell layers and both the nasal and lung epithelia in balb/c mice. the progeny viruses and expressed p24 were released mainly from apical surfaces. in balb/c mice, the infection was limited to the respiratory system and was not observed in the blood. this showed that polarized distribution limited antigen delivery into the whole body and thus limited immune response. to see if this could be improved upon, we stimulated unpolarized budding of the virus and hiv antigens by treating both caco-2 cells and balb/c mice with colchicine. we found that, in balb/c mice, the degree of infection and antigen expression in the epithelia went up. as a result, specific immune responses increased correspondingly. together, these data suggest that polarized budding limits antigen delivery and immune responses, but unpolarized distribution can increase antigen expression and delivery and thus enhance specific immune responses. this conclusion can be used to optimize mucosal hiv vaccine strategies.
WOS关键词	POLARIZED EPITHELIAL-CELLS ; HUMAN AIRWAY EPITHELIA ; IMMUNODEFICIENCY-VIRUS ; BASOLATERAL SURFACE ; TIANTAN VACCINIA ; SEX WORKERS ; INFECTION ; ENTRY ; VACCINATION ; IMMUNITY
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000295041700024
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375953
专题	武汉病毒研究所
通讯作者	Zhang, Yan
作者单位	1.Chinese Acad Sci, Mucosal Immun Res Grp, State Key Lab Virol, Wuhan Inst Virol, Wuhan, Peoples R China 2.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yan,Yang, Jingyi,Bao, Rong,et al. Unpolarized release of vaccinia virus and hiv antigen by colchicine treatment enhances intranasal hiv antigen expression and mucosal humoral responses[J]. Plos one,2011,6(9):13.
APA	Zhang, Yan.,Yang, Jingyi.,Bao, Rong.,Chen, Yaoqing.,Zhou, Dihan.,...&Yan, Huimin.(2011).Unpolarized release of vaccinia virus and hiv antigen by colchicine treatment enhances intranasal hiv antigen expression and mucosal humoral responses.Plos one,6(9),13.
MLA	Zhang, Yan,et al."Unpolarized release of vaccinia virus and hiv antigen by colchicine treatment enhances intranasal hiv antigen expression and mucosal humoral responses".Plos one 6.9(2011):13.

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