| Novel bacillus thuringiensis delta-endotoxin active against locusta migratoria manilensis | |
| Wu, Yan; Lei, Cheng-Feng; Yi, Dan; Liu, Peng-Ming; Gao, Mei-Ying | |
| 刊名 | Applied and environmental microbiology
 |
| 2011-05-01 | |
| 卷号 | 77期号:10页码:3227-3233 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/aem.02462-10 |
| 通讯作者 | Gao, mei-ying(mygao@wh.iov.cn) |
| 英文摘要 | A novel delta-endotoxin gene was cloned from a bacillus thuringiensis strain with activity against locusta migratoria manilensis by pcr-based genome walking. the sequence of the cry gene was 3,432 bp long, and it encoded a cry protein of 1,144 amino acid residues with a molecular mass of 129,196.5 kda, which exhibited 62% homology with cry7ba1 in the amino acid sequence. the delta-endotoxin with five conserved sequence blocks in the amino-terminal region was designated cry7ca1 (genbank accession no. ef486523). protein structure analysis suggested that the activated toxin of cry7ca1 has three domains: 227 residues forming 7 alpha-helices (domain i); 213 residues forming three antiparallel beta-sheets (domain ii); and 134 residues forming a beta-sandwich (domain iii). the three domains, respectively, exhibited 47, 44, and 34% sequence identity with corresponding domains of known cry toxins. sds-page and western blot analysis showed that cry7ca1, encoded by the full-length open reading frame of the cry gene, the activated toxin 1, which included three domains but without the n-terminal 54 amino acid residues and the c terminus, and the activated toxin 2, which included three domains and n-terminal 54 amino acid residues but without the c terminus, could be expressed in escherichia coli. bioassay results indicated that the expressed proteins of cry7ca1 and the activated toxins (toxins 1 and 2) showed significant activity against 2nd instar locusts, and after 7 days of infection, the estimated 50% lethal concentrations (lc(50)s) were 8.98 mu g/ml for the expressed cry7ca1, 0.87 mu g/ml for the activated toxin 1, and 4.43 mu g/ml for the activated toxin 2. the delta-endotoxin also induced histopathological changes in midgut epithelial cells of adult l. migratoria manilensis. |
| WOS关键词 | INSECTICIDAL CRYSTAL PROTEINS ; PORE-FORMING DOMAIN ; PARASPORAL INCLUSIONS ; HELIOTHIS-VIRESCENS ; BOMBYX-MORI ; TOXIN ; STRAINS ; CELLS ; DIVERSITY ; BINDING |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| 出版者 | AMER SOC MICROBIOLOGY |
| WOS记录号 | WOS:000290473200009 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375862 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Mei-Ying |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yan,Lei, Cheng-Feng,Yi, Dan,et al. Novel bacillus thuringiensis delta-endotoxin active against locusta migratoria manilensis[J]. Applied and environmental microbiology,2011,77(10):3227-3233. |
| APA | Wu, Yan,Lei, Cheng-Feng,Yi, Dan,Liu, Peng-Ming,&Gao, Mei-Ying.(2011).Novel bacillus thuringiensis delta-endotoxin active against locusta migratoria manilensis.Applied and environmental microbiology,77(10),3227-3233. |
| MLA | Wu, Yan,et al."Novel bacillus thuringiensis delta-endotoxin active against locusta migratoria manilensis".Applied and environmental microbiology 77.10(2011):3227-3233. |
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