| Characterization of the nonstructural protein ns80 of grass carp reovirus | |
| Fan, Chao1,2; Shao, Ling1,2; Fang, Qin1 | |
| 刊名 | Archives of virology
 |
| 2010-11-01 | |
| 卷号 | 155期号:11页码:1755-1763 |
| ISSN号 | 0304-8608 |
| DOI | 10.1007/s00705-010-0753-6 |
| 通讯作者 | Fang, qin(qfang@wh.iov.cn) |
| 英文摘要 | Nonstructural proteins of members of the family reoviridae are believed to play significant roles in the virus replication cycle. phylogenetic analyses indicate that the nonstructural protein ns80 of grass carp reovirus, encoded by a gene of segment 4 (s4), is a primary determinant that is related to the formation of viroplasmic inclusion bodies (vib), where viral replication and assembly are thought to occur. to understand the role of the ns80 protein in viral replication, an initial investigation of ns80 gene expression in both infected and transfected cells was conducted. transmission electron microscopy results indicate that replication and assembly of gcrv occur within vib-like structures in the perinuclear region of the cell cytoplasm. furthermore, expression of the s4 gene in infected cells was detected with an ns80-specific antibody by western blot and immunofluorescence. moreover, globular vib-like structures were observed when expressing gfp-derived full-length ns80 (pegfp-c1/ns80) and recombinants containing the c-terminal conserved region (pegfp-c1/ns80(335-724)) in transfected vero. no such structures were detected in cells transfected with an n-terminal recombinant (pegfp-c1/ns80(1-334)), suggesting that the ns80 c-terminal conserved region may be involved in the formation of inclusion structures. these data provide a foundation for further functional studies of ns80 related to viral inclusion formation in viral replication. |
| WOS关键词 | FACTORY-LIKE INCLUSIONS ; MU-NS ; FAMILY REOVIRIDAE ; GENUS AQUAREOVIRUS ; GENOME SEGMENTS ; CORE PARTICLES ; SIGMA-NS ; VIRUS ; IDENTIFICATION ; CELLS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | SPRINGER WIEN |
| WOS记录号 | WOS:000284238700002 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375766 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Fang, Qin |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Chao,Shao, Ling,Fang, Qin. Characterization of the nonstructural protein ns80 of grass carp reovirus[J]. Archives of virology,2010,155(11):1755-1763. |
| APA | Fan, Chao,Shao, Ling,&Fang, Qin.(2010).Characterization of the nonstructural protein ns80 of grass carp reovirus.Archives of virology,155(11),1755-1763. |
| MLA | Fan, Chao,et al."Characterization of the nonstructural protein ns80 of grass carp reovirus".Archives of virology 155.11(2010):1755-1763. |
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