| Protection against multiple influenza a virus subtypes by intranasal administration of recombinant nucleoprotein | |
| Guo, Lina1; Zheng, Mei1; Ding, Yahong1; Li, Dongmei1; Yang, Zhongdong1; Wang, Haiming1; Chen, Quanjiao2; Sui, Zhiwei2; Fang, Fang3; Chen, Ze1,2,3 | |
| 刊名 | Archives of virology
 |
| 2010-11-01 | |
| 卷号 | 155期号:11页码:1765-1775 |
| ISSN号 | 0304-8608 |
| DOI | 10.1007/s00705-010-0756-3 |
| 通讯作者 | Chen, ze(chenze2005@263.net) |
| 英文摘要 | Vaccination is a cost-effective way to control the influenza epidemic. vaccines based on highly conserved antigens can provide protection against different influenza a strains and subtypes. in this study, the recombinant nucleoprotein (rnp) of the a/pr/8/34 (h1n1) influenza virus strain was effectively expressed using a prokaryotic expression system and then purified with a nickel-charged sepharose affinity column as a candidate component for an influenza vaccine. the rnp was administered intranasally three times at 3-week intervals to female balb/c mice in combination with an adjuvant (cholera toxin b subunit containing 0.2% of the whole toxin). twenty-one days after the last immunization, the mice were challenged with homologous or heterologous influenza viruses at a lethal dose. the results showed that intranasal immunization of 10 mu g rnp with adjuvant completely protected the immunized mice against the homologous influenza virus, and immunization with 100 mu g rnp in combination with adjuvant provided good cross-protection against heterologous h5n1 and h9n2 avian influenza viruses. the results indicate that such a vaccine administered intranasally can induce mucosal and cell-mediated immunity, thus having the potential to control epidemics caused by new emerging influenza viruses. |
| WOS关键词 | EXPRESSING PLASMID DNAS ; IMMUNOGLOBULIN-A ; INTRACELLULAR NEUTRALIZATION ; H5N1 INFLUENZA ; VIRAL PROTEIN ; HETEROSUBTYPIC IMMUNITY ; MEDIATED PROTECTION ; HIV-1 REPLICATION ; MICE ; INFECTION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | SPRINGER WIEN |
| WOS记录号 | WOS:000284238700003 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375744 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Ze |
| 作者单位 | 1.Shanghai Inst Biol Prod, Shanghai 200052, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 3.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Lina,Zheng, Mei,Ding, Yahong,et al. Protection against multiple influenza a virus subtypes by intranasal administration of recombinant nucleoprotein[J]. Archives of virology,2010,155(11):1765-1775. |
| APA | Guo, Lina.,Zheng, Mei.,Ding, Yahong.,Li, Dongmei.,Yang, Zhongdong.,...&Chen, Ze.(2010).Protection against multiple influenza a virus subtypes by intranasal administration of recombinant nucleoprotein.Archives of virology,155(11),1765-1775. |
| MLA | Guo, Lina,et al."Protection against multiple influenza a virus subtypes by intranasal administration of recombinant nucleoprotein".Archives of virology 155.11(2010):1765-1775. |
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