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	Physiological and biochemical characterization of sheepgrass (leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions
	Li, Lingyu1,2; Yang, Haomeng1; Ren, Weibo3; Liu, Bei1,2; Cheng, Dongmei1,2; Wu, Xinhong3; Gong, Jirui4; Peng, Lianwei1; Huang, Fang1
刊名	Journal of plant biology
	2016-08-01
卷号	59期号:4页码:336-346
关键词	Leymus chinensis Phosphate starvation Photosystem ii Ros-scavenging enzymes Thylakoid membranes 2d-bn/page
ISSN号	1226-9239
DOI	10.1007/s12374-016-0117-1
通讯作者	Huang, fang(fhuang@ibcas.ac.cn)
英文摘要	Sheepgrass [leymus chinensis (trin.) tzvel] is a valuble forage plant highly significant to regional grassland productivity of euro-asia steppes. although effects of environmental stress including drought have been studied, impact of nutrient deficiency in particular phosphate (pi), one of the essential macronutrient, is not reported. here, we investigated low-pi effect on its photosynthetic apparatus via physiological and biochemical analysis. we show that psii activity was significantly reduced based on chlorophyll fluorescence measurements. we observed decreased amount of core proteins of psii by immunoblot analysis. further analysis of thylakoid membranes using 2d-bn/sds-page and immunoblot detection demonstrated that the amount of psii complexes was closely correlated with the pi levels within the range. together with reduced number of thylakoid grana stackings observed, we suggest that the maintenance of psii is impaired under pi-limited condition. moreover, enzyme activity assays revealed that the activity of several ros scavenger enzymes was stimulated by low-pi treatment. based on these experimental results we conclude that psii is the component of photosynthetic apparatus most sensitive to pi supply and the enhanced anti-ros activity is mainly subjective to protection of psii against low pi-induced photo-oxidative stress in the organism.
WOS关键词	PHOTOOXIDATIVE STRESS ; MOLECULAR-MECHANISMS ; PHOSPHORUS-NUTRITION ; LIPID-PEROXIDATION ; PHOTOSYSTEM-II ; GLYCINE-MAX ; ARABIDOPSIS ; PLANTS ; TOLERANCE ; ASSAY
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000381137700004
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375637
专题	中国科学院大学
通讯作者	Huang, Fang
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China 4.Beijing Normal Univ, Coll Resources Sci & Technol, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Li, Lingyu,Yang, Haomeng,Ren, Weibo,et al. Physiological and biochemical characterization of sheepgrass (leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions[J]. Journal of plant biology,2016,59(4):336-346.
APA	Li, Lingyu.,Yang, Haomeng.,Ren, Weibo.,Liu, Bei.,Cheng, Dongmei.,...&Huang, Fang.(2016).Physiological and biochemical characterization of sheepgrass (leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions.Journal of plant biology,59(4),336-346.
MLA	Li, Lingyu,et al."Physiological and biochemical characterization of sheepgrass (leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions".Journal of plant biology 59.4(2016):336-346.

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