QQ客服 官方微博 反馈留言

	Functional studies of per os infectivity factors of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus
	Song, Jingjiao; Wang, Ranran; Deng, Fei; Wang, Hualin; Hu, Zhihong1
刊名	Journal of general virology
	2008-09-01
卷号	89页码:2331-2338
ISSN号	0022-1317
DOI	10.1099/vir.0.2008/002352-0
通讯作者	Hu, zhihong(huzh@wh.iov.cn)
英文摘要	A combined functional investigation on the four per os infectivity factors (pifs) of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (hearnpv) was conducted in this study. hearnpv bacmids with deletions of p74 (ha20), pif1 (ha111), pif2 (ha132) and pif3 (ha98) were constructed individually by homologous recombination in escherichia coli cells. repaired bacmids with respective pifs were also constructed. western blot analyses revealed that all four pifs were structural components of the envelope of hearnpv occlusion-derived virus (odv). electron microscopy showed that deletion of the pifs did not have any obvious effects on the morphology of the occlusion bodies (obs). bioassay analyses indicated that deletion of any of the above pifs resulted in loss of oral infectivity of obs. the mixtures of the four pif-deletion mutants also resulted in deficiency of oral infectivity, implying that the four pifs must be structural components of the same odv to accomplish their function. repairing of the respective genes into the pif-deletion bacmids could rescue the oral infectivity of the pit-deletion viruses. calcofluor, which can damage the peritrophic membrane (pm), could not rescue the defects of the oral infectivity of the pif-deletion viruses, indicating that the pm is not likely to be the functional target of the pifs.
WOS关键词	OCCLUSION-DERIVED VIRUS ; HELIOTHIS-VIRESCENS LARVAE ; NUCLEAR POLYHEDROSIS-VIRUS ; ENVELOPE FUSION PROTEIN ; BACULOVIRUS ; P74 ; MIDGUT ; ENTRY ; IDENTIFICATION ; INSECTS
WOS研究方向	Biotechnology & Applied Microbiology ; Virology
WOS类目	Biotechnology & Applied Microbiology ; Virology
语种	英语
出版者	SOC GENERAL MICROBIOLOGY
WOS记录号	WOS:000259285600030
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375515
专题	武汉病毒研究所
通讯作者	Hu, Zhihong
作者单位	1.Chinese Acad Sci, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Song, Jingjiao,Wang, Ranran,Deng, Fei,et al. Functional studies of per os infectivity factors of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus[J]. Journal of general virology,2008,89:2331-2338.
APA	Song, Jingjiao,Wang, Ranran,Deng, Fei,Wang, Hualin,&Hu, Zhihong.(2008).Functional studies of per os infectivity factors of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.Journal of general virology,89,2331-2338.
MLA	Song, Jingjiao,et al."Functional studies of per os infectivity factors of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus".Journal of general virology 89(2008):2331-2338.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。