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	Immortalizaton of swine umbilical vein endothelial cells with human telomerase reverse transcriptase
	Hong, Hai Xia1; Zhang, Yan Ming1; Xu, Hao1; Su, Zheng Yuan2; Sun, Pei1
刊名	Molecules and cells
	2007-12-31
卷号	24期号:3页码:358-363
关键词	Human telomerase reverse transcriptase (htert) Immortalization Liposome Positive clone Swine umbilical vein endothelial cells (suvecs) Transfection
ISSN号	1016-8478
通讯作者	Zhang, yan ming(ylzhangym@sohu.com)
英文摘要	Swine endothelial cells are commonly used as an in vitro model for studying features of the blood-brain barrier and some hemorrhagic diseases. however, primary cultures of swine cells have finite lifespans. to establish immortalized swine umbilical vein endothelial cells (suvecs) using human telomerase reverse transcriptase (htert), the plasmid pci-neo-htert was transfected into suvecs by lipofection. clones were selected for 6418 resistance, and positive clones were amplified. one of the clones was cultured for up to 50 passages. factor viii-related antigen and cd34 were detected. the immortalized cells shared the properties of normal cells, such as contact inhibition, serum requirement and anchorage dependence. karyotype analysis revealed that the immortalized cells were in the diploid range. in addition, both in vivo and in vitro assays of tumorigenicity showed no neoplastic transformation. furthermore, no, pgi(2), and et-1 concentrations in the transfected cells were normal. these results suggest that the suvecs immortalized by htert retain their original characteristics.
WOS关键词	IN-VITRO ; CATALYTIC SUBUNIT ; HTERT GENE ; T-ANTIGEN ; EXPRESSION ; TRANSFECTION ; FIBROBLASTS ; SENESCENCE ; EXTENSION
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
WOS记录号	WOS:000252248000008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375410
专题	武汉病毒研究所
通讯作者	Zhang, Yan Ming
作者单位	1.NW A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Dept Mol Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Hong, Hai Xia,Zhang, Yan Ming,Xu, Hao,et al. Immortalizaton of swine umbilical vein endothelial cells with human telomerase reverse transcriptase[J]. Molecules and cells,2007,24(3):358-363.
APA	Hong, Hai Xia,Zhang, Yan Ming,Xu, Hao,Su, Zheng Yuan,&Sun, Pei.(2007).Immortalizaton of swine umbilical vein endothelial cells with human telomerase reverse transcriptase.Molecules and cells,24(3),358-363.
MLA	Hong, Hai Xia,et al."Immortalizaton of swine umbilical vein endothelial cells with human telomerase reverse transcriptase".Molecules and cells 24.3(2007):358-363.

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