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	A novel therapeutic approach to 6-ohda-induced parkinson's disease in rats via supplementation of ptd-conjugated tyrosine hydroxylase
	Wu, SP; Fu, AL; Wang, YX; Yu, LP; Jia, PY; Li, Q; Jin, GZ; Sun, MJ
刊名	Biochemical and biophysical research communications
	2006-07-21
卷号	346期号:1页码:1-6
关键词	Tyrosine hydroxylase Protein transduction domain 6-hydroxydopamine Parkinson's disease
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2006.03.247
通讯作者	Sun, mj()
英文摘要	The present study aimed to evaluate whether the protein transduction domain (ptd)-conjugated human tyrosine hydroxylase (th) fusion protein was effective on the 6-hydroxydopamine (6-ohda)-induced parkinson's disease (pd) model rats. an expression vector pet-ptd-th harbouring the ptd-th gene was constructed and transformed to the escherichia coli bl21 cells for expression. the expressed recombinant ptd-th with a molecular weight of 61 kd was successfully transduced (1 mu m) into the dopaminergic sh-sy5y human neuroblastoma cells in vitro and visualized by immunohistochemical assay. an in vivo experiment in rats showed that the iv administered ptd-th protein (8 mg/kg) permeated across the blood-brain barrier, penetrated into the striatum and midbrain, and peaked at 5-8 h after the injection. the behavioral effects of ptd-th on the apomorphine-induced rotations in the pd model rats ;8 weeks after the 6-ohda lesion showed that a single bolus of ptd-th (8 mg/kg) iv injection caused a decrement of 60% of the contralateral turns on day 1 and 40% on days 5-17. the results imply that iv delivery of ptd-th is therapeutically effective on the 6-ohda-induced pd in rats, the ptd-mediated human th treatment opening a promising therapeutic direction in treatment of pd. (c) 2006 elsevier inc. all rights reserved.
WOS关键词	PROTEIN TRANSDUCTION ; HIV-1 TAT ; CHOLINE-ACETYLTRANSFERASE ; BASIC DOMAIN ; DELIVERY ; CELLS ; GENE
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000238549100001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375323
专题	武汉病毒研究所
通讯作者	Sun, MJ
作者单位	1.Acad Mil Med Sci, Dept Biochem & Pharmacol, Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China 2.Beijing Ctr Dis Prevent & Control, Beijing 100013, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Drug Res, Dept Pharmacol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wu, SP,Fu, AL,Wang, YX,et al. A novel therapeutic approach to 6-ohda-induced parkinson's disease in rats via supplementation of ptd-conjugated tyrosine hydroxylase[J]. Biochemical and biophysical research communications,2006,346(1):1-6.
APA	Wu, SP.,Fu, AL.,Wang, YX.,Yu, LP.,Jia, PY.,...&Sun, MJ.(2006).A novel therapeutic approach to 6-ohda-induced parkinson's disease in rats via supplementation of ptd-conjugated tyrosine hydroxylase.Biochemical and biophysical research communications,346(1),1-6.
MLA	Wu, SP,et al."A novel therapeutic approach to 6-ohda-induced parkinson's disease in rats via supplementation of ptd-conjugated tyrosine hydroxylase".Biochemical and biophysical research communications 346.1(2006):1-6.

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