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	Open reading frame 132 of heliocoverpa armigera nucleopolyhedrovirus encodes a functional per os infectivity factor (pif-2)
	Fang, Minggang; Nie, Yingchao; Wang, Qian; Deng, Fei; Wang, Ranran; Wang, Hanzhong; Wang, Hualin; Vlak, Just M.; Chen, Xinwen; Hu, Zhihong
刊名	Journal of general virology
	2006-09-01
卷号	87页码:2563-2569
ISSN号	0022-1317
DOI	10.1099/vir.0.81788-0
通讯作者	Hu, zhihong(huzh@wh.iov.cn)
英文摘要	Open reading frame 132 (ha132) of helicoverpa armigera nucleopolyhedrovirus (hearnpv) is a homologue of per os infectivity factor 2 (pif-2) of spodoptera exigua multiple nucleopolyhedrovirus. sequence analysis indicated that ha132 encoded a protein of 383 aa with a predicted molecular mass of 44.5 kda. alignment of ha132 and its baculovirus homologues revealed that ha132 was highly conserved among baculoviruses, with 14 absolutely conserved cysteine residues. rt-pcr indicated that ha132 was first transcribed at 24 h post-infection. western blot analysis showed that a 43 kda band was detectable in hearnpv-infected hzam1 cells from 36 in post-infection. western blots also indicated that ha132 was a component of the occlusion-derived virus, but not of budded virus. deletion of ha132 from hearnpv abolished per os infectivity, but had no effect on the infectivity of the budded virus phenotype.
WOS关键词	OCCLUSION-DERIVED VIRUS ; SINGLE-NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; AUTOGRAPHA-CALIFORNICA NUCLEOPOLYHEDROVIRUS ; NUCLEAR POLYHEDROSIS-VIRUS ; HELIOTHIS-VIRESCENS LARVAE ; ENVELOPE PROTEIN P74 ; MULTIPLE NUCLEOPOLYHEDROVIRUS ; ORGYIA-PSEUDOTSUGATA ; NUCLEOTIDE-SEQUENCE ; HELICOVERPA-ZEA
WOS研究方向	Biotechnology & Applied Microbiology ; Virology
WOS类目	Biotechnology & Applied Microbiology ; Virology
语种	英语
出版者	SOC GENERAL MICROBIOLOGY
WOS记录号	WOS:000239939900013
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375277
专题	武汉病毒研究所
通讯作者	Hu, Zhihong
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 3.Wageningen Univ, Dept Virol, NL-6709 PD Wageningen, Netherlands
推荐引用方式 GB/T 7714	Fang, Minggang,Nie, Yingchao,Wang, Qian,et al. Open reading frame 132 of heliocoverpa armigera nucleopolyhedrovirus encodes a functional per os infectivity factor (pif-2)[J]. Journal of general virology,2006,87:2563-2569.
APA	Fang, Minggang.,Nie, Yingchao.,Wang, Qian.,Deng, Fei.,Wang, Ranran.,...&Hu, Zhihong.(2006).Open reading frame 132 of heliocoverpa armigera nucleopolyhedrovirus encodes a functional per os infectivity factor (pif-2).Journal of general virology,87,2563-2569.
MLA	Fang, Minggang,et al."Open reading frame 132 of heliocoverpa armigera nucleopolyhedrovirus encodes a functional per os infectivity factor (pif-2)".Journal of general virology 87(2006):2563-2569.

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