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	The key dna-binding residues in the c-terminal domain of mycobacterium tuberculosis dna gyrase a subunit (gyra)
	Huang, You-Yi; Deng, Jiao-Yu; Gu, Jing; Zhang, Zhi-Ping; Maxwell, Anthony; Bi, Li-Jun; Chen, Yuan-Yuan; Zhou, Ya-Feng; Yu, Zi-Niu; Zhang, Xian-En
刊名	Nucleic acids research
	2006-11-01
卷号	34期号:19页码:5650-5659
ISSN号	0305-1048
DOI	10.1093/nar/gkl695
通讯作者	Zhang, xian-en(zhangxe@most.cn)
英文摘要	As only the type ii topoisomerase is capable of introducing negative supercoiling, dna gyrase is involved in crucial cellular processes. although the other domains of dna gyrase are better understood, the mechanism of dna binding by the c-terminal domain of the dna gyrase a subunit (gyra-ctd) is less clear. here, we investigated the dna-binding sites in the gyra-ctd of mycobacterium tuberculosis gyrase through site-directed mutagenesis. the results show that y577, r691 and r745 are among the key dna-binding residues in m.tuberculosis gyra-ctd, and that the third blade of the gyra-ctd is the main dna-binding region in m.tuberculosis dna gyrase. the substitutions of y577a, d669a, r691a, r745a and g729w led to the loss of supercoiling and relaxation activities, although they had a little effect on the drug-dependent dna cleavage and decatenation activities, and had no effect on the atpase activity. taken together, these results showed that the gyra-ctd is essential to dna gyrase of m.tuberculosis, and promote the idea that the m.tuberculosis gyra-ctd is a new potential target for drug design. it is the first time that the dna-binding sites in gyra-ctd have been identified.
WOS关键词	BREAKAGE-REUNION DOMAIN ; ESCHERICHIA-COLI ; CRYSTAL-STRUCTURE ; B-PROTEIN ; NOVOBIOCIN RESISTANCE ; ATPASE REACTION ; MUTATION ; TOPOISOMERASES ; FRAGMENT ; ENZYME
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000241955200040
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375269
专题	武汉病毒研究所
通讯作者	Zhang, Xian-En
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Biophys, State Key Lab Macromol, Beijing 100101, Peoples R China 3.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agromicrobiol, Wuhan 430070, Peoples R China 4.John Innes Ctr, Dept Biol Chem, Norwich NR4 7UH, Norfolk, England
推荐引用方式 GB/T 7714	Huang, You-Yi,Deng, Jiao-Yu,Gu, Jing,et al. The key dna-binding residues in the c-terminal domain of mycobacterium tuberculosis dna gyrase a subunit (gyra)[J]. Nucleic acids research,2006,34(19):5650-5659.
APA	Huang, You-Yi.,Deng, Jiao-Yu.,Gu, Jing.,Zhang, Zhi-Ping.,Maxwell, Anthony.,...&Zhang, Xian-En.(2006).The key dna-binding residues in the c-terminal domain of mycobacterium tuberculosis dna gyrase a subunit (gyra).Nucleic acids research,34(19),5650-5659.
MLA	Huang, You-Yi,et al."The key dna-binding residues in the c-terminal domain of mycobacterium tuberculosis dna gyrase a subunit (gyra)".Nucleic acids research 34.19(2006):5650-5659.

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