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	Surface-enhanced infrared absorption spectroscopy and electrochemistry reveal the impact of nanoparticles on the function of protein immobilized on mimic biointerface
	Zhang, Xiaofei1,2; Zeng, Li1,2; Liu, Li1; Ma, Zhifang1,2; Jiang, Xiue1,2
刊名	Electrochimica acta
	2016-09-01
卷号	211页码:148-155
关键词	Cytochrome c Sio2 nanoparticles Electron transfer Surface-enhanced infrared adsorption spectroscopy
ISSN号	0013-4686
DOI	10.1016/j.electacta.2016.06.037
通讯作者	Jiang, xiue(jiangxiue@ciac.ac.cn)
英文摘要	Study of the interactions between engineered nanoparticles and biomolecules plays an important role in understanding nano-bio effect. however, most of studies focus on the interaction of protein with nanoparticle in bulk phase, the impact of nanoparticle on the function of protein immobilized on the biological interface has been ignored for a long time. in this paper, we study the effect of sio2 nps on electron transfer function of cytochrome c (cyt c) adsorbed onto the 11-mercaptoundecanoic acid (mua) self-assembled monolayer (sam) by surface-enhanced infrared absorption spectroscopy (seiras) combined with electrochemistry. our results disclose that instead of inhibiting the electron transfer, exposure of sio2 nps enhances the redox reversibility, electron transfer rate, and catalytic performance of adsorbed cyt c, but induces a slight negative shift in the formal potential of the adsorbed cyt c. interaction of sio2 nps slightly induces the structural changes of the beta-sheet in the oxidized form and the change of microenvironment surrounding them, which could further change the microenvironment and the orientation of heme, facilitating the hydration of cyt c at the oxidized state. it could be the enhanced hydration of cyt c (fe3+) and a smaller change in the total hydration from reduced to oxidized state that results in the slight negative shift in the formal potential and promotes the electron transfer capability. besides, cyt c's slight conformational change and the disturbance to the microenvironment of heme resulting from the adsorption of sio2 nps could also be one of the reason that leads to the enhanced catalytic capability towards the reduction of h2o2. (c) 2016 elsevier ltd. all rights reserved.
WOS关键词	CYTOCHROME-C ; ELECTRON-TRANSFER ; SILICA NANOPARTICLES ; MOLECULAR-STRUCTURE ; PEROXIDASE-ACTIVITY ; GOLD ELECTRODE ; REDOX PROCESS ; ADSORPTION ; MONOLAYER ; CORONA
WOS研究方向	Electrochemistry
WOS类目	Electrochemistry
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000380904100018
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375230
专题	中国科学院大学
通讯作者	Jiang, Xiue
作者单位	1.Chinese Acad Sci, State Key Lab Electroanalyt Chem, Changchun Inst Appl Chem, Changchun 130022, Jilin, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaofei,Zeng, Li,Liu, Li,et al. Surface-enhanced infrared absorption spectroscopy and electrochemistry reveal the impact of nanoparticles on the function of protein immobilized on mimic biointerface[J]. Electrochimica acta,2016,211:148-155.
APA	Zhang, Xiaofei,Zeng, Li,Liu, Li,Ma, Zhifang,&Jiang, Xiue.(2016).Surface-enhanced infrared absorption spectroscopy and electrochemistry reveal the impact of nanoparticles on the function of protein immobilized on mimic biointerface.Electrochimica acta,211,148-155.
MLA	Zhang, Xiaofei,et al."Surface-enhanced infrared absorption spectroscopy and electrochemistry reveal the impact of nanoparticles on the function of protein immobilized on mimic biointerface".Electrochimica acta 211(2016):148-155.

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