| A label-free microelectrode array based on one-step synthesis of chitosan-multi-walled carbon nanotube-thionine for ultrasensitive detection of carcinoembryonic antigen | |
| Xu, Huiren1,2; Wang, Yang1,2; Wang, Li1,2; Song, Yilin1,2; Luo, Jinping1,2; Cai, Xinxia1,2 | |
| 刊名 | Nanomaterials
 |
| 2016-07-01 | |
| 卷号 | 6期号:7页码:12 |
| 关键词 | Multi-walled carbon nanotube Microelectrode array Carcinoembryonic antigen One-step synthesis Label-free |
| ISSN号 | 2079-4991 |
| DOI | 10.3390/nano6070132 |
| 通讯作者 | Cai, xinxia(xxcai@mail.ie.ac.cn) |
| 英文摘要 | Carcinoembryonic antigen (cea) has been an extensively used tumor marker responsible for clinical early diagnosis of cervical carcinomas, and pancreatic, colorectal, gastric and lung cancer. combined with micro-electro mechanical system (mems) technology, it is important to develop a novel immune microelectrode array (mea) not only for rapid analysis of serum samples, but also for cell detection in vitro and in vivo. in this work, we depict a simple approach to modify chitosan-multi-walled carbon nanotubes-thionine (cs-mwcnts-thi) hybrid film through one-step electrochemical deposition and the cs-mwcnts-thi hybrid films are successfully employed to immobilize anti-cea for fabricating simple, label-free, and highly sensitive electro-chemical immune meas. the detection principle of immune mea was based on the fact that the increasing formation of the antigen-antibody immunocomplex resulted in the decreased response currents and the relationship between the current reductions with the corresponding cea concentrations was directly proportional. experimental results indicated that the label-free mea had good selectivity and the limit of detection for cea is 0.5 pg/ml signal to noise ratio (snr) = 3. a linear calibration plot for the detection of cea was obtained in a wide concentration range from 1 pg/ml to 100 ng/ml (r = 0.996). this novel mea has potential applications for detecting cea for the research on cancer cells and cancer tissue slices as well as for effective early diagnosis. |
| WOS关键词 | SENSITIVE ELECTROCHEMICAL IMMUNOASSAY ; THYROID-STIMULATING HORMONE ; FREE IMMUNOSENSOR ; PRUSSIAN BLUE ; NANOCOMPOSITES ; GRAPHENE ; CEA ; NANOPARTICLES ; OPPORTUNITIES ; BIOMATERIALS |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | MDPI AG |
| WOS记录号 | WOS:000380769100013 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375118 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cai, Xinxia |
| 作者单位 | 1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Huiren,Wang, Yang,Wang, Li,et al. A label-free microelectrode array based on one-step synthesis of chitosan-multi-walled carbon nanotube-thionine for ultrasensitive detection of carcinoembryonic antigen[J]. Nanomaterials,2016,6(7):12. |
| APA | Xu, Huiren,Wang, Yang,Wang, Li,Song, Yilin,Luo, Jinping,&Cai, Xinxia.(2016).A label-free microelectrode array based on one-step synthesis of chitosan-multi-walled carbon nanotube-thionine for ultrasensitive detection of carcinoembryonic antigen.Nanomaterials,6(7),12. |
| MLA | Xu, Huiren,et al."A label-free microelectrode array based on one-step synthesis of chitosan-multi-walled carbon nanotube-thionine for ultrasensitive detection of carcinoembryonic antigen".Nanomaterials 6.7(2016):12. |
| 个性服务 |
| 查看访问统计 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论