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	Sigma(e)-dependent activation of rbpa controls transcription of the fura-katg operon in response to oxidative stress in mycobacteria
	Hu, Yangbo1; Wang, Zhongwei1,2; Feng, Lipeng1,2; Chen, Zhenkang1,2; Mao, Chunyou1,2; Zhu, Yan1,2; Chen, Shiyun1
刊名	Molecular microbiology
	2016-10-01
卷号	102期号:1页码:107-120
ISSN号	0950-382X
DOI	10.1111/mmi.13449
通讯作者	Chen, shiyun(sychen@wh.iov.cn)
英文摘要	Mycobacterium tuberculosis adopts various strategies to cope with oxidative stress during infection. transcriptional regulators, including sigma factors, make important contributions to this stress response, but how these proteins cooperate with each other is largely unknown. in this study, the role of rbpa and its cooperation with sigma factors in response to oxidative stress are investigated. knock down expression of rbpa in mycobacterium smegmatis attenuated bacterial survival in the presence of h2o2. additionally, transcription of the rbpa gene was induced by h2o2 in a sigma(e)-dependent manner. after induction, rbpa interacts with the principal sigma factor, sigma(a), to control the transcription of fura-katg operon, which encodes an h2o2 scavenging enzyme. moreover, this regulation is responsible for the role of sigma(e) in oxidative response because bacterial survival was attenuated and transcription of the fura-katg operon was down-regulated with h2o2 treatment in sige deletion mutant (sige), and over-expression of rbpa in sige strain restored all of these phenotypes. taken together, our study first illustrated a mechanism for sigma(e) in response to oxidative stress through regulation of rbpa transcription. this study was also the first to demonstrate that rbpa is required for the full response to oxidative stress by cooperating with the principal sigma(a).
WOS关键词	SIGMA-FACTOR ; RNA-POLYMERASE ; BINDING PROTEIN ; STREPTOMYCES-COELICOLOR ; FLP RECOMBINASE ; GENE-EXPRESSION ; IN-VIVO ; TUBERCULOSIS ; SMEGMATIS ; SURVIVAL
WOS研究方向	Biochemistry & Molecular Biology ; Microbiology
WOS类目	Biochemistry & Molecular Biology ; Microbiology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000384807900008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375051
专题	中国科学院大学
通讯作者	Chen, Shiyun
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10086, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yangbo,Wang, Zhongwei,Feng, Lipeng,et al. Sigma(e)-dependent activation of rbpa controls transcription of the fura-katg operon in response to oxidative stress in mycobacteria[J]. Molecular microbiology,2016,102(1):107-120.
APA	Hu, Yangbo.,Wang, Zhongwei.,Feng, Lipeng.,Chen, Zhenkang.,Mao, Chunyou.,...&Chen, Shiyun.(2016).Sigma(e)-dependent activation of rbpa controls transcription of the fura-katg operon in response to oxidative stress in mycobacteria.Molecular microbiology,102(1),107-120.
MLA	Hu, Yangbo,et al."Sigma(e)-dependent activation of rbpa controls transcription of the fura-katg operon in response to oxidative stress in mycobacteria".Molecular microbiology 102.1(2016):107-120.

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