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	Characterization and expression of a novel caspase gene: evidence of the expansion of caspases in crassostrea gigas
	Li, Zhongxiao1,2; Wang, Chao1; Jiang, Fengjuan1,2; Huan, Pin1; Liu, Baozhong1,3
刊名	Comparative biochemistry and physiology b-biochemistry & molecular biology
	2016-11-01
卷号	201页码:37-45
关键词	Crassostrea gigas Caspase Thermal stress Apoptosis
ISSN号	1096-4959
DOI	10.1016/j.cbpb.2016.07.001
通讯作者	Liu, baozhong(bzliu@qdio.ac.cn)
英文摘要	Caspases are a group of cysteine-aspartate proteases involved in apoptosis and a variety of non-apoptotic processes. in this study, a novel caspase gene was cloned and its potential role in apoptosis was investigated. the caspase gene (cgcasp 3/7) has an open reading frame of 1626 bp encoding 541 amino acids containing the conserved functional domains and motifs of effector caspases. its amino acid sequence shows low identity with the other effector caspases of crassostrea gigas and contains a unique long intersubunit linker (il). the cgcasp 3/7 mrna was expressed highly in oocytes and then decreased gradually after fertilization, indicating cgcasp 3/7 could function in oocyte apoptosis. in adult tissues, it is located primarily in the gills and hepatopancreas. we examined the mrna expression of cgcasp 3/7 in gills of oysters immersed in ambient (17 degrees c) or heated (27 degrees c) seawater. the thermal stress stimulated mrna expression of cgcasp 3/7 by 2.5- and 4.1-fold at 2 h and 6 h post-treatment, respectively, indicating cgcasp3/7 was involved in the early response to thermal stress. to examine the function of the il, cgcasp 3/7 and cgcasp 3/7-t (with a truncated il) were expressed using an in vitro translation system and their devdase activity was measured. both proteins showed a significantly higher level of devdase activity than control, but cgcasp3/7-t had lower devdase activity than cgcasp3/7, indicating cgcasp3/7 had devdase activity and the il was required for maximal devdase activity. our study adds to the complexity of caspases in c gigas. (c) 2016 elsevier inc. all rights reserved.
WOS关键词	PACIFIC OYSTER ; SUMMER MORTALITY ; THERMAL-STRESS ; APOPTOSIS ; ACTIVATION ; MARKER
WOS研究方向	Biochemistry & Molecular Biology ; Zoology
WOS类目	Biochemistry & Molecular Biology ; Zoology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000385330500005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374757
专题	中国科学院大学
通讯作者	Liu, Baozhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
推荐引用方式 GB/T 7714	Li, Zhongxiao,Wang, Chao,Jiang, Fengjuan,et al. Characterization and expression of a novel caspase gene: evidence of the expansion of caspases in crassostrea gigas[J]. Comparative biochemistry and physiology b-biochemistry & molecular biology,2016,201:37-45.
APA	Li, Zhongxiao,Wang, Chao,Jiang, Fengjuan,Huan, Pin,&Liu, Baozhong.(2016).Characterization and expression of a novel caspase gene: evidence of the expansion of caspases in crassostrea gigas.Comparative biochemistry and physiology b-biochemistry & molecular biology,201,37-45.
MLA	Li, Zhongxiao,et al."Characterization and expression of a novel caspase gene: evidence of the expansion of caspases in crassostrea gigas".Comparative biochemistry and physiology b-biochemistry & molecular biology 201(2016):37-45.

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