| A dna binding winged helix domain in caf-1 functions with pcna to stabilize caf-1 at replication forks | |
| Zhang, Kuo1,2; Gao, Yuan3; Li, Jingjing1; Burgess, Rebecca3; Han, Junhong4,5,6; Liang, Huanhuan1; Zhang, Zhiguo3; Liu, Yingfang1 | |
| 刊名 | Nucleic acids research
 |
| 2016-06-20 | |
| 卷号 | 44期号:11页码:5083-5094 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkw106 |
| 通讯作者 | Zhang, zhiguo(zhang.zhiguo@mayo.edu) ; Liu, yingfang(liuy@ibp.ac.cn) |
| 英文摘要 | Chromatin assembly factor 1 (caf-1) is a histone h3-h4 chaperone that deposits newly synthesized histone (h3-h4)(2) tetramers during replication-coupled nucleosome assembly. however, how caf-1 functions in this process is not yet well understood. here, we report the crystal structure of c terminus of cac1 (cac1c), a subunit of yeast caf-1, and the function of this domain in stabilizing caf-1 at replication forks. we show that cac1c forms a winged helix domain (whd) and binds dna in a sequence-independent manner. mutations in cac1c that abolish dna binding result in defects in transcriptional silencing and increased sensitivity to dna damaging agents, and these defects are exacerbated when combined with cac1 mutations deficient in pcna binding. similar phenotypes are observed for corresponding mutations in mouse caf-1. these results reveal a mechanism conserved in eukaryotic cells whereby the ability of caf-1 to bind dna is important for its association with the dna replication forks and subsequent nucleosome assembly. |
| WOS关键词 | ASSEMBLY FACTOR-I ; NUCLEOSOME CORE PARTICLE ; CRYSTAL-STRUCTURE ; S-PHASE ; (H3-H4)(2) TETRAMERS ; ANGSTROM RESOLUTION ; HISTONE DEPOSITION ; STRAND-SEPARATION ; STRUCTURAL BASIS ; MOUSE CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000379753100020 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2374557 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Zhiguo; Liu, Yingfang |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, State Key Lab Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Mayo Clin, Dept Biochem & Mol Biol, Rochester, MN 55905 USA 4.Sichuan Univ, State Key Lab Biotherapy, Chengdu 610041, Peoples R China 5.Sichuan Univ, West China Hosp, Div Abdominal Canc, Ctr Canc, Chengdu 610041, Peoples R China 6.Natl Collaborat Innovat Ctr Biotherapy, Chengdu 610041, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Kuo,Gao, Yuan,Li, Jingjing,et al. A dna binding winged helix domain in caf-1 functions with pcna to stabilize caf-1 at replication forks[J]. Nucleic acids research,2016,44(11):5083-5094. |
| APA | Zhang, Kuo.,Gao, Yuan.,Li, Jingjing.,Burgess, Rebecca.,Han, Junhong.,...&Liu, Yingfang.(2016).A dna binding winged helix domain in caf-1 functions with pcna to stabilize caf-1 at replication forks.Nucleic acids research,44(11),5083-5094. |
| MLA | Zhang, Kuo,et al."A dna binding winged helix domain in caf-1 functions with pcna to stabilize caf-1 at replication forks".Nucleic acids research 44.11(2016):5083-5094. |
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