| Comparative characterization of two gdp-mannose dehydrogenase genes from saccharina japonica (laminariales, phaeophyceae) | |
| Zhang,Pengyan1,2,3; Shao,Zhanru1,2; Jin,Weihua1,2; Duan,Delin1,2,4 | |
| 刊名 | Bmc plant biology
 |
| 2016-03-08 | |
| 卷号 | 16期号:1 |
| 关键词 | Saccharina japonica Alginate Gdp-mannose dehydrogenase Enzyme kinetics Stress response |
| ISSN号 | 1471-2229 |
| DOI | 10.1186/s12870-016-0750-3 |
| 通讯作者 | Duan,delin(dlduan@qdio.ac.cn) |
| 英文摘要 | Abstractbackgroundsaccharina japonica is an important commercial brown seaweed, its main product is alginate, which is used in food, textile and by the cosmetic and pharmaceutical industries. gdp-mannose dehydrogenase (gmd) is the key enzyme involved in the synthesis of alginate. however, little is known about gmd in s. japonica. here we report comparative biochemical analysis of two gmd genes in s. japonica.resultstwo gmd genes from s. japonica (sjgmd1, sjgmd2) were cloned. the open reading frame lengths of sjgmd1, sjgmd2 are 963?bp and 948?bp, respectively. alignment analysis showed that the two sjgmd sequences shared 79.38?% identity. both proteins possess the ggxclpkdv and gxgxvg sequence motifs characteristic of the short-chain dehydrogenase/reductase superfamily. the optimum temperatures for sjgmds were 30?°c (sjgmd1) and 20?°c (sjgmd2), and the optimum ph values were 8.0 (sjgmd1) and 8.25 (sjgmd2). kinetic analysis demonstrated the km values for the substrate gdp-mannose were 289?μm (sjgmd1) and 177?μm (sjgmd2), and the km values for the cofactor nad+ were 139?μm (sjgmd1) and 195?μm (sjgmd2). the metal iron zn2+ is a potent inhibitor of sjgmd1 and sjgmd2. real-time pcr analysis showed that heat and desiccation treatments resulted in a significant increase in sjgmd1 and sjgmd2 transcript abundance, suggesting that the sjgmds are directly involved in the acclimitisation of s. japonica to abiotic stresses.conclusionour work identified two novel genes encoding gmd in s. japonica, comparatively characterized their structural characteristics and enzyme kinetics, and revealed the function of gmd in the stress adaptability of s. japonica. the knowledge obtained here enriched our understanding of the alginate synthesis mechanism in s. japonica, and may promote further research on functional differences between gmd genes. |
| 语种 | 英语 |
| 出版者 | BioMed Central |
| WOS记录号 | BMC:10.1186/S12870-016-0750-3 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2374317 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Duan,Delin |
| 作者单位 | 1.Chinese Academy of Sciences; Key Laboratory of Experimental Marine Biology, Institute of Oceanology 2.Qingdao National Laboratory for Marine Science and Technology; Laboratory for Marine Biology and Biotechnology 3.University of Chinese Academy of Sciences 4.State Key Laboratory of Seaweed Bioactive Substances |
| 推荐引用方式 GB/T 7714 | Zhang,Pengyan,Shao,Zhanru,Jin,Weihua,et al. Comparative characterization of two gdp-mannose dehydrogenase genes from saccharina japonica (laminariales, phaeophyceae)[J]. Bmc plant biology,2016,16(1). |
| APA | Zhang,Pengyan,Shao,Zhanru,Jin,Weihua,&Duan,Delin.(2016).Comparative characterization of two gdp-mannose dehydrogenase genes from saccharina japonica (laminariales, phaeophyceae).Bmc plant biology,16(1). |
| MLA | Zhang,Pengyan,et al."Comparative characterization of two gdp-mannose dehydrogenase genes from saccharina japonica (laminariales, phaeophyceae)".Bmc plant biology 16.1(2016). |
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