| Rapid detection of viral antibodies based on multifunctional staphylococcus aureus nanobioprobes | |
| Qiao, Jinjuan1,2; Li, Yunpeng1,2; Wei, Cuihua1,2; Yang, Hang1; Yu, Junping1; Wei, Hongping1 | |
| 刊名 | Enzyme and microbial technology
 |
| 2016-12-01 | |
| 卷号 | 95页码:94-99 |
| 关键词 | Virus Antibody detection Staphylococcus aureus 5-cyano-2,3-ditolyltetrazolium chloride |
| ISSN号 | 0141-0229 |
| DOI | 10.1016/j.enzmictec.2016.09.006 |
| 通讯作者 | Wei, hongping(hpwei@wh.iov.cn) |
| 英文摘要 | Biosynthesis of nanoparticles inside s.aureus cells has enhanced the sensitivity of immunoassays based on the s. aureus nanoparticles. however, the current methods are limited to antigen detection by conjugating igg antibodies on s. aureus nanoparticles. in this study, a simple way to conjugate antigens to the s. aureus nanobioparticles was developed by utilizing a cell wall binding domain (cbd) from a bacteriophage lysin plyv12. based on this novel design, simple agglutination tests of the igg antibodies of ebola virus (ebov) nucleoprotein (np) and middle east respiratory virus (mers) np in rabbit sera were successfully developed by conjugating the s. aureus nanobioparticles with two fusion proteins ebov np- cbd and mers np-cbd, respectively. the conjugation was done easily by just mixing the fusion proteins with the s. aureus nanoparticles. the detection time was within 20 min without any special equipment or expertise. as far as we know, this is the first time to realize the detection of viral antibodies based on s. aureus nanoparticles. (c) 2016 elsevier inc. all rights reserved. |
| WOS关键词 | LINKED-IMMUNOSORBENT-ASSAY ; EBOLA-VIRUS ; RECOMBINANT NUCLEOPROTEIN ; IMMUNOFLUORESCENCE METHOD ; NEUTRALIZING ANTIBODIES ; PATHOGEN DETECTION ; IMMUNOGLOBULIN-G ; HELA-CELLS ; MERS-COV ; CORONAVIRUS |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE INC |
| WOS记录号 | WOS:000389161400010 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373492 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wei, Hongping |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Emerging Pathogens & Infect, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Jinjuan,Li, Yunpeng,Wei, Cuihua,et al. Rapid detection of viral antibodies based on multifunctional staphylococcus aureus nanobioprobes[J]. Enzyme and microbial technology,2016,95:94-99. |
| APA | Qiao, Jinjuan,Li, Yunpeng,Wei, Cuihua,Yang, Hang,Yu, Junping,&Wei, Hongping.(2016).Rapid detection of viral antibodies based on multifunctional staphylococcus aureus nanobioprobes.Enzyme and microbial technology,95,94-99. |
| MLA | Qiao, Jinjuan,et al."Rapid detection of viral antibodies based on multifunctional staphylococcus aureus nanobioprobes".Enzyme and microbial technology 95(2016):94-99. |
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