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	Mtase domain of dendrolimus punctatus cypovirus vp3 mediates virion attachment and interacts with host alp protein
	Su, Lan1,2; Xu, Congrui1,2; Cheng, Chuangang1; Lei, Chengfeng1; Sun, Xiulian1
刊名	Viruses-basel
	2017-04-01
卷号	9期号:4页码:13
关键词	Dpcpv Attachment proteins Alp Viral entry
ISSN号	1999-4915
DOI	10.3390/v9040066
通讯作者	Sun, xiulian(sunxl@wh.iov.cn)
英文摘要	Dendrolimus punctatus cypovirus (dpcpv) is an important pathogen of d. punctatus, but little is known about the mechanisms of dpcpv infection. here, we investigated the effects of vp3, vp4 and vp5 structural proteins on the viral invasion. both the c-terminal of vp3 (methyltransferase (mtase) domain) and vp4 (a-spike) bound to spodoptera exigua midgut brush border membrane vesicles (bbmvs) in a dose-dependent manner, and the binding was inhibited by purified dpcpv virions. importantly, anti-mtase and anti-vp4 antibodies inhibited viral binding to s. exigua bbmvs. using far-western blots, a 65 kda protein in bombyx mori bbmvs, identified as alkaline phosphatase protein (bmalp) by mass spectrometry, specifically interacted with dpcpv mtase. the interaction between mtase and bmalp was verified by co-immunoprecipitation in vitro. pretreatment of b. mori bbmvs with an anti-alp antibody or incubation of dpcpv virions with prokaryotically expressed bmalp reduced viral attachment. additionally, bmalp inhibited dpcpv infection in s. exigua larvae. our data provide evidence that the mtase domain and a-spike function as viral attachment proteins during the dpcpv infection process, and alp is the ligand that interacts with dpcpv via the mtase domain. these results augment our understanding of the mechanisms used by cypoviruses to enter their hosts.
WOS关键词	CYTOPLASMIC-POLYHEDROSIS-VIRUS ; ENTEROVIRUS 71 ; ALKALINE-PHOSPHATASE ; ATOMIC MODEL ; CELL ENTRY ; RECEPTOR ; MECHANISM ; TOXIN ; IDENTIFICATION ; REOVIRUSES
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000404224400005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373420
专题	武汉病毒研究所
通讯作者	Sun, Xiulian
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Su, Lan,Xu, Congrui,Cheng, Chuangang,et al. Mtase domain of dendrolimus punctatus cypovirus vp3 mediates virion attachment and interacts with host alp protein[J]. Viruses-basel,2017,9(4):13.
APA	Su, Lan,Xu, Congrui,Cheng, Chuangang,Lei, Chengfeng,&Sun, Xiulian.(2017).Mtase domain of dendrolimus punctatus cypovirus vp3 mediates virion attachment and interacts with host alp protein.Viruses-basel,9(4),13.
MLA	Su, Lan,et al."Mtase domain of dendrolimus punctatus cypovirus vp3 mediates virion attachment and interacts with host alp protein".Viruses-basel 9.4(2017):13.

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