| Ceruloplasmin inhibits the production of extracellular hepatitis b virions by targeting its middle surface protein | |
| Zhao, Kaitao1,2; Wu, Chunchen1; Yao, Yongxuan1,2; Cao, Liang1,2; Zhang, Zhenhua3,4; Yuan, Yifei1,2; Wang, Yun1; Pei, Rongjuan1; Chen, Jizheng1; Hu, Xue1 | |
| 刊名 | Journal of general virology
 |
| 2017-06-01 | |
| 卷号 | 98期号:6页码:1410-1421 |
| 关键词 | Hepatitis b virus Virion production Ceruloplasmin Middle surface protein |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/jgv.0.000794 |
| 通讯作者 | Wu, chunchen(wucc@wh.iov.cn) |
| 英文摘要 | Ceruloplasmin (cp) is mainly synthesized by hepatocytes and plays an essential role in iron metabolism. previous reports have shown that cp levels correlate negatively with disease progression in patients with chronic hepatitis b. however, the function of cp in the hepatitis b virus (hbv) life cycle and the mechanism underlying the above correlation remain unclear. here, we report that cp can selectively inhibit the production of extracellular hbv virions without altering intracellular viral replication. hbv expression can also downregulate the expression of cp. knockdown of cp using small interfering rna significantly increased the level of extracellular hbv virions in both huh7 and hepg2.2.15 cells, while overexpression of cp decreased this level. mechanistically, cp could specifically interact with the hbv middle surface protein (mhb). using an hbv replication-competent clone unable to express mhbs, we demonstrated that the overexpression of cp did not affect the production of extracellular hbv virions in the absence of mhbs. furthermore, introduction of an mhb expression construct could rescue the impairment in virion production caused by cp. taken together, our results suggest that cp may be an important host factor that targets mhbs during the envelopment and/or release of virions. |
| WOS关键词 | AMINO-ACID SUBSTITUTIONS ; N-LINKED GLYCOSYLATION ; HUMAN SERUM-ALBUMIN ; HEPATOCELLULAR-CARCINOMA ; PARTICLE MORPHOGENESIS ; VIRUS REPLICATION ; ENVELOPE PROTEIN ; LIVER-FAILURE ; ANTIGEN HBSAG ; EXPRESSION |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| 出版者 | MICROBIOLOGY SOC |
| WOS记录号 | WOS:000410020900030 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373372 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wu, Chunchen |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Anhui Med Univ, Affiliated Hosp 1, Dept Infect Dis, Hefei 230022, Anhui, Peoples R China 4.Anhui Med Univ, Sch Pharm, Hefei 230022, Anhui, Peoples R China 5.Univ Hosp Essen, Inst Virol, Essen, Germany |
| 推荐引用方式 GB/T 7714 | Zhao, Kaitao,Wu, Chunchen,Yao, Yongxuan,et al. Ceruloplasmin inhibits the production of extracellular hepatitis b virions by targeting its middle surface protein[J]. Journal of general virology,2017,98(6):1410-1421. |
| APA | Zhao, Kaitao.,Wu, Chunchen.,Yao, Yongxuan.,Cao, Liang.,Zhang, Zhenhua.,...&Chen, Xinwen.(2017).Ceruloplasmin inhibits the production of extracellular hepatitis b virions by targeting its middle surface protein.Journal of general virology,98(6),1410-1421. |
| MLA | Zhao, Kaitao,et al."Ceruloplasmin inhibits the production of extracellular hepatitis b virions by targeting its middle surface protein".Journal of general virology 98.6(2017):1410-1421. |
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