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	Human norovirus ns3 has rna helicase and chaperoning activities
	Li, Teng-Feng1,2; Hosmillo, Myra3; Schwanke, Hella3; Shu, Ting1,2; Wang, Zhaowei1,2; Yin, Lei2; Curry, Stephen4; Goodfellow, Ian G.3; Zhou, Xi1,2
刊名	Journal of virology
	2018-03-01
卷号	92期号:5页码:18
关键词	Rna chaperone Rna helicase Norovirus
ISSN号	0022-538X
DOI	10.1128/jvi.01606-17
通讯作者	Zhou, xi(zhouxi@wh.iov.cn)
英文摘要	Rna-remodeling proteins, including rna helicases and chaperones, act to remodel rna structures and/or protein-rna interactions and are required for all processes involving rnas. although many viruses encode rna helicases and chaperones, their in vitro activities and their roles in infected cells largely remain elusive. noroviruses are a diverse group of positive-strand rna viruses in the family caliciviridae and constitute a significant and potentially fatal threat to human health. here, we report that the protein ns3 encoded by human norovirus has both atp-dependent rna helicase activity that unwinds rna helices and atp-independent rna-chaperoning activity that can remodel structured rnas and facilitate strand annealing. moreover, ns3 can facilitate viral rna synthesis in vitro by norovirus polymerase. ns3 may therefore play an important role in norovirus rna replication. lastly, we demonstrate that the rna-remodeling activity of ns3 is inhibited by guanidine hydrochloride, an fda-approved compound, and, more importantly, that it reduces the replication of the norovirus replicon in cultured human cells. altogether, these findings are the first to demonstrate the presence of rna-remodeling activities encoded by caliciviridae and highlight the functional significance of ns3 in the noroviral life cycle. importance noroviruses are a diverse group of positive-strand rna viruses, which annually cause hundreds of millions of human infections and over 200,000 deaths worldwide. for rna viruses, cellular or virus-encoded rna helicases and/or chaperones have long been considered to play pivotal roles in viral life cycles. however, neither rna helicase nor chaperoning activity has been demonstrated to be associated with any norovirus-encoded proteins, and it is also unknown whether norovirus replication requires the participation of any viral or cellular rna helicases/chaperones. we found that a norovirus protein, ns3, not only has atp-dependent helicase activity, but also acts as an atp-independent rna chaperone. also, ns3 can facilitate in vitro viral rna synthesis, suggesting the important role of ns3 in norovirus replication. moreover, ns3 activities can be inhibited by an fda-approved compound, which also suppresses norovirus replicon replication in human cells, raising the possibility that ns3 could be a target for antinoroviral drug development.
WOS关键词	EATON-LAMBERT SYNDROME ; NORWALK-LIKE VIRUSES ; NUCLEOSIDE TRIPHOSPHATASE ; BIOLOGICAL-ACTIVITIES ; REPLICATIVE HELICASE ; GUANIDINE COMPOUNDS ; PROTEIN 2C ; CELL-LINE ; POLIOVIRUS ; GENOME
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000424744800004
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373337
专题	武汉病毒研究所
通讯作者	Zhou, Xi
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 2.Wuhan Univ, Coll Life Sci, Wuhan, Hubei, Peoples R China 3.Univ Cambridge, Addenbrookes Hosp, Dept Pathol, Div Virol, Cambridge, England 4.Imperial Coll London, Dept Life Sci, London, England
推荐引用方式 GB/T 7714	Li, Teng-Feng,Hosmillo, Myra,Schwanke, Hella,et al. Human norovirus ns3 has rna helicase and chaperoning activities[J]. Journal of virology,2018,92(5):18.
APA	Li, Teng-Feng.,Hosmillo, Myra.,Schwanke, Hella.,Shu, Ting.,Wang, Zhaowei.,...&Zhou, Xi.(2018).Human norovirus ns3 has rna helicase and chaperoning activities.Journal of virology,92(5),18.
MLA	Li, Teng-Feng,et al."Human norovirus ns3 has rna helicase and chaperoning activities".Journal of virology 92.5(2018):18.

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