| Dcaf1 is involved in hcv replication through regulation of mir-122 | |
| Yan, Yanling1,2; Li, Conghui1,2; Sun, Binlian1; Yang, Rongge1 | |
| 刊名 | Archives of virology
 |
| 2018-04-01 | |
| 卷号 | 163期号:4页码:977-985 |
| ISSN号 | 0304-8608 |
| DOI | 10.1007/s00705-017-3691-8 |
| 通讯作者 | Sun, binlian(sunbl@wh.iov.cn) ; Yang, rongge(ryang@wh.iov) |
| 英文摘要 | Hepatitis c virus (hcv) is a worldwide threaten to human health with a high ratio of chronic infections. recently, we found that vpr-mediated regulation of hcv replication depends on the host protein ddb1-cul4 associate factor 1 (dcaf1), implying that dcaf1 might be involved in the replication of hcv. in this study, we demonstrated that dcaf1 knockdown reduced hcv replication both in the infectious (jfh1) and replicon (con1) systems. further investigation showed a negative regulation of hcv internal ribosome entry site (ires)-mediated translation by dcaf1. considering the positive effects on the replication of the hcv replicon, we speculated that dcaf1 affected the balance between hcv rna replication and protein translation. since mir-122 is involved in the regulation of this balance, we investigated the influence of dcaf1 on mir-122 expression. by measuring the expression of mir-122, pre-mir-122 and its target cat-1 mrna, we found that mir-122 was downregulated following dcaf1 knockdown. furthermore, overexpression of mir-122 rescued hcv replication impairment induced by dcaf1 knockdown. in conclusion, our study suggests that dcaf1 is involved in hcv replication through regulation of mir-122 and thus provides new insights into the interaction between hcv and the host cell. |
| WOS关键词 | HEPATITIS-C VIRUS ; 5' UNTRANSLATED REGION ; CELL-CULTURE ; PROTEIN VPR ; RNA REPLICATION ; TRANSLATION ; INFECTION ; EPIDEMIOLOGY ; DEGRADATION ; BINDING |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | SPRINGER WIEN |
| WOS记录号 | WOS:000427657200015 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373315 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Sun, Binlian; Yang, Rongge |
| 作者单位 | 1.Chinese Acad Sci, Res Grp HIV Mol Epidemiol & Virol, Ctr Mol Virol, State Key Lab Virol,Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Yanling,Li, Conghui,Sun, Binlian,et al. Dcaf1 is involved in hcv replication through regulation of mir-122[J]. Archives of virology,2018,163(4):977-985. |
| APA | Yan, Yanling,Li, Conghui,Sun, Binlian,&Yang, Rongge.(2018).Dcaf1 is involved in hcv replication through regulation of mir-122.Archives of virology,163(4),977-985. |
| MLA | Yan, Yanling,et al."Dcaf1 is involved in hcv replication through regulation of mir-122".Archives of virology 163.4(2018):977-985. |
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