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	The group i alphabaculovirus-specific protein, ac5, is a novel component of the occlusion body but is not associated with odvs or the pif complex
	Wang, Xi1,2; Chen, Cheng1,2; Zhang, Nan1,2; Li, Jiang1; Deng, Fei1; Wang, Hualin1; Vlak, Just M.3; Hu, Zhihong1; Wang, Manli1
刊名	Journal of general virology
	2018-04-01
卷号	99期号:4页码:585-595
关键词	Baculovirus Ac5 Function Pif complex Occlusion body Group i alphabaculovirus
ISSN号	0022-1317
DOI	10.1099/jgv.0.001031
通讯作者	Hu, zhihong(huzh@wh.iov.cn) ; Wang, manli(wangml@wh.iov.cn)
英文摘要	Autographa californica nucleopolyhedrovirus (acmnpv) orf5 (ac5) is a group i alphabaculovirus-specific gene of unknown function, although the protein (ac5) was previously reported to be associated with the per os infectivity factor (pif) complex. the purpose of this study was to study the dynamics of ac5 during acmnpv infection and to verify whether it is indeed a component of the pif complex. transcription and expression analyses suggested that ac5 is a late viral gene. an ac5-deleted recombinant acmnpv was generated by homologous recombination. a one-step growth curve assay indicated that ac5 was not required for budded virus (by) production in sf9 cells. scanning electron microscopy and transmission electron microscopy demonstrated that the deletion of ac5 did not affect occlusion body (ob) morphology, and nor did it affect the insertion of occlusion-derived virus (odv) into obs. partially denaturing sds-page and a co-immunoprecipitation assay clearly showed that ac5 was not a component of the pif complex, while the deletion of ac5 did not affect the formation and presence of the pif complex. further analyses showed, however, that ac5 was an ob-specific protein, but it was not detected as a component of bvs or odvs. bioassay experiments showed that the oral infectivity of ac5-deleted acmnpv to third instar spodoptera exigua larvae was not significantly different from that of the ac5-repaired virus. in conclusion, ac5 is an intrinsic protein of obs, instead of being a component of the pif complex, and is not essential for either bv or odv infection. ac5 is awaiting the assignment of another hitherto unknown function.
WOS关键词	PER-OS INFECTIVITY ; CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS ; ENVELOPE FUSION PROTEIN ; INNER NUCLEAR-MEMBRANE ; VIRUS ENVELOPE ; SORTING MOTIF ; BUDDED-VIRUS ; BACULOVIRUS ; GENE ; ENCODES
WOS研究方向	Biotechnology & Applied Microbiology ; Virology
WOS类目	Biotechnology & Applied Microbiology ; Virology
语种	英语
出版者	MICROBIOLOGY SOC
WOS记录号	WOS:000431022600016
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373269
专题	武汉病毒研究所
通讯作者	Hu, Zhihong; Wang, Manli
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol & Joint Lab Invertebrate Viro, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Wageningen Univ & Res, Lab Virol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands
推荐引用方式 GB/T 7714	Wang, Xi,Chen, Cheng,Zhang, Nan,et al. The group i alphabaculovirus-specific protein, ac5, is a novel component of the occlusion body but is not associated with odvs or the pif complex[J]. Journal of general virology,2018,99(4):585-595.
APA	Wang, Xi.,Chen, Cheng.,Zhang, Nan.,Li, Jiang.,Deng, Fei.,...&Wang, Manli.(2018).The group i alphabaculovirus-specific protein, ac5, is a novel component of the occlusion body but is not associated with odvs or the pif complex.Journal of general virology,99(4),585-595.
MLA	Wang, Xi,et al."The group i alphabaculovirus-specific protein, ac5, is a novel component of the occlusion body but is not associated with odvs or the pif complex".Journal of general virology 99.4(2018):585-595.

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