| Hearnpv pseudotyped with pif1, 2, and 3 from mabrnpv: infectivity and complex stability | |
| Makalliwa, George Alliwa1,2,3; Wang, Xi1,3; Zhang, Huanyu1,3; Zhang, Nan1,3; Chen, Cheng1,3; Li, Jiang1; Deng, Fei1; Wang, Hualin1; Wang, Manli1; Hu, Zhihong1 | |
| 刊名 | Virologica sinica
 |
| 2018-04-01 | |
| 卷号 | 33期号:2页码:187-196 |
| 关键词 | Baculovirus Oral infectivity Per os infectivity factors (pifs) Pif complex Pseudotyped |
| ISSN号 | 1674-0769 |
| DOI | 10.1007/s12250-018-0014-5 |
| 通讯作者 | Wang, manli(wangml@wh.iov.cn) ; Hu, zhihong(huzh@wh.iov.cn) |
| 英文摘要 | Effective oral infection is set off by interaction of a group of conserved per os infectivity factors (pifs) with larval midgut columnar epithelial cells. we constructed pseudotyped viruses by substituting pif1, pif2 or pif3 genes of helicoverpa armigera nucleopolyhedrovirus (hearnpv) with their homologs from mamestra bracissae multiple nucleopolyhedrovirus and tested their infectivity to tissue culture cells and to larvae. transfection and infection assays revealed that all recombinant viruses generated infectious budded virus in both cell culture and in larvae. electron microscopy showed synthesized occlusion body and occlusion derived virus (odv) were morphologically indistinguishable from those of the parental virus. by contrast, feeding assays revealed that pseudotyped viruses could not rescue oral infectivity except for pif3 pseudotyped virus that only partially rescued oral infectivity but at a mortality rate much lower than that of the parental hearnpv. consistent with the bioassay result, pif complex was detected in odvs of pif3 pseudotyped virus only but not in pif1 or pif2 pseudotyped viruses. our results suggest that pif complex is essential for oral infectivity, and in the formation of the pif complex, pif1, 2 are virus-specific while pif3 does not appear to be as specific and can function in heterologous environment, albeit to a much more limited extent. |
| WOS关键词 | PER-OS INFECTIVITY ; OCCLUSION-DERIVED VIRUS ; SINGLE-NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; CALIFORNICA-M NUCLEOPOLYHEDROVIRUS ; PROTEIN-PROTEIN INTERACTIONS ; NUCLEAR POLYHEDROSIS-VIRUS ; BACULOVIRUS ; IDENTIFICATION ; REVEALS ; LARVAE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000431413000009 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373268 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Manli; Hu, Zhihong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Jomo Kenyatta Univ Agr & Technol, POB 62000-00200, Nairobi, Kenya 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Makalliwa, George Alliwa,Wang, Xi,Zhang, Huanyu,et al. Hearnpv pseudotyped with pif1, 2, and 3 from mabrnpv: infectivity and complex stability[J]. Virologica sinica,2018,33(2):187-196. |
| APA | Makalliwa, George Alliwa.,Wang, Xi.,Zhang, Huanyu.,Zhang, Nan.,Chen, Cheng.,...&Hu, Zhihong.(2018).Hearnpv pseudotyped with pif1, 2, and 3 from mabrnpv: infectivity and complex stability.Virologica sinica,33(2),187-196. |
| MLA | Makalliwa, George Alliwa,et al."Hearnpv pseudotyped with pif1, 2, and 3 from mabrnpv: infectivity and complex stability".Virologica sinica 33.2(2018):187-196. |
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