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	Cellular-beacon-mediated counting for the ultrasensitive detection of ebola virus on an integrated micromagnetic platform
	Hong, Shao-Li1; Zhang, Ya-Nan1; Liu, Ya-Hua1; Tang, Man1; Pang, Dai-Wen1; Wong, Gary2,3; Chen, Jianjun4; Qiu, Xiangguo5; Gao, George F.2,3; Liu, Wenjun3
刊名	Analytical chemistry
	2018-06-19
卷号	90期号:12页码:7310-7317
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b00513
通讯作者	Bi, yuhai(beeyh@im.ac.cn) ; Zhang, zhi-ling(zlzhang@whu.edu.cn)
英文摘要	Ebola virus (ebov) disease is a complex zoonosis that is highly virulent in humans and has caused many deaths. highly sensitive detection of ebov is of great importance for early-stage diagnosis for increasing the probability of survival. herein, we established a cellular-beacon-mediated counting strategy for an ultrasensitive ebov assay on a micromagnetic platform. the detection platform, which was assisted by on-demand magnetic-field manipulation, showed high integration and enhanced complex-sample pretreatment by magnetophoretic separation and continuous-flow washing. cellular beacons (i.e., fluorescent cells) with superior optical properties were used as reporters, and each cellular beacon was used as a fluorescent tracking unit to quantify ebov by counting the numbers of individual fluorescent signals on the micromagnetic platform. this method achieves high sensitivity with a detection limit as low as 2.6 pg/ml, and the detection limit shows little difference in a complex matrix. in addition, it has excellent specificity and good reproducibility. these results indicate that this method proposes an ultrasensitive detection strategy for early diagnosis of the disease.
WOS关键词	POINT-OF-CARE ; IMMUNOMAGNETIC SEPARATION ; HEMORRHAGIC-FEVER ; MAGNETIC-FIELD ; NANOSENSOR ; CAPTURE ; ELISA ; CHIP ; AMPLIFICATION ; NANOPARTICLES
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000436028800032
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373256
专题	武汉病毒研究所
通讯作者	Bi, Yuhai; Zhang, Zhi-Ling
作者单位	1.Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China 2.Shenzhen Third Peoples Hosp, Shenzhen Key Lab Pathogen & Immun, State Key Discipline Infect Dis, Shenzhen 518112, Peoples R China 3.Chinese Acad Sci, Ctr Influenza Res & Early Warning CASCIRE, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Virol, key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 5.Publ Hlth Agcy Canada, Natl Microbiol Lab, Special Pathogens Program, Winnipeg, MB R3E 3R2, Canada
推荐引用方式 GB/T 7714	Hong, Shao-Li,Zhang, Ya-Nan,Liu, Ya-Hua,et al. Cellular-beacon-mediated counting for the ultrasensitive detection of ebola virus on an integrated micromagnetic platform[J]. Analytical chemistry,2018,90(12):7310-7317.
APA	Hong, Shao-Li.,Zhang, Ya-Nan.,Liu, Ya-Hua.,Tang, Man.,Pang, Dai-Wen.,...&Zhang, Zhi-Ling.(2018).Cellular-beacon-mediated counting for the ultrasensitive detection of ebola virus on an integrated micromagnetic platform.Analytical chemistry,90(12),7310-7317.
MLA	Hong, Shao-Li,et al."Cellular-beacon-mediated counting for the ultrasensitive detection of ebola virus on an integrated micromagnetic platform".Analytical chemistry 90.12(2018):7310-7317.

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