| Isolation of a novel bacillus thuringiensis phage representing a new phage lineage and characterization of its endolysin | |
| Yuan, Yihui1,2; Peng, Qin3; Yang, Shuo1; Zhang, Shaowen1; Fu, Yajuan2; Wu, Yan2; Gao, Meiying2 | |
| 刊名 | Viruses-basel
 |
| 2018-11-01 | |
| 卷号 | 10期号:11页码:13 |
| 关键词 | Bacteriophage Endolysin Restriction modification system Bacillus thuringiensis Tadpole-like phage virion |
| ISSN号 | 1999-4915 |
| DOI | 10.3390/v10110611 |
| 通讯作者 | Gao, meiying(mygao@wh.iov.cn) |
| 英文摘要 | Phages, the parasites of bacteria, are considered as a new kind of antimicrobial agent due to their ability to lyse pathogenic bacteria. due to the increase of available phage isolates, the newly isolated phage showed increasing genomic similarities with previously isolated phages. in this study, the novel phage vb_bths_bmbphi, infecting the bacillus thuringiensis strain bmb171, is isolated and characterized together with its endolysin. this phage is the first tadpole-like phage infecting the bacillus strains. genomic analysis shows that the phage genome is dissimilar to all those of previously characterized phages, only exhibiting low similarities with partial regions of the b. thuringiensis prophages. phylogenetic analysis revealed that the phage was distant from the other bacillus phages in terms of evolution. the novel genome sequence, the distant evolutionary relationship, and the special virion morphology together suggest that the phage vb_bths_bmbphi could be classified as a new phage lineage. the genome of the phage is found to contain a restriction modification system, which might endow the phage with immunity to the restriction modification system of the host bacterium. the function of the endolysin plybmb encoded by the phage vb_bths_bmbphi was analyzed, and the endolysin could lyse all the tested bacillus cereus group strains, suggesting that the endolysin might be used in controlling pathogenic b. cereus group strains. the findings of this study enrich the understanding of phage diversity and provide a resource for controlling the b. cereus group pathogenic bacteria. |
| WOS关键词 | GENOMIC ANALYSIS ; SEQUENCE ; BACTERIOPHAGES ; ANTHRACIS ; PROTEINS ; ENDONUCLEASES ; GENETICS ; CEREUS ; STRAIN |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000451657600034 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373215 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Meiying |
| 作者单位 | 1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 3.Hainan Normal Univ, Coll Life Sci, Minist Educ, Key Lab Ecol Trop Isl, Haikou 571158, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Yihui,Peng, Qin,Yang, Shuo,et al. Isolation of a novel bacillus thuringiensis phage representing a new phage lineage and characterization of its endolysin[J]. Viruses-basel,2018,10(11):13. |
| APA | Yuan, Yihui.,Peng, Qin.,Yang, Shuo.,Zhang, Shaowen.,Fu, Yajuan.,...&Gao, Meiying.(2018).Isolation of a novel bacillus thuringiensis phage representing a new phage lineage and characterization of its endolysin.Viruses-basel,10(11),13. |
| MLA | Yuan, Yihui,et al."Isolation of a novel bacillus thuringiensis phage representing a new phage lineage and characterization of its endolysin".Viruses-basel 10.11(2018):13. |
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