| Rapid detection of banna virus by reverse transcription-loop -mediated isothermal amplification (rt-lamp) | |
| Xia, Han1; Zhao, Na1,2; Zhao, Lu1,2; Wang, Yujuan3; Zhao, Wang4; Yuan, Zhiming1 | |
| 刊名 | International journal of infectious diseases
 |
| 2019 | |
| 卷号 | 78页码:93-98 |
| 关键词 | Banna virus Rt-lamp Mosquito Detection |
| ISSN号 | 1201-9712 |
| DOI | 10.1016/j.ijid.2018.10.025 |
| 通讯作者 | Xia, han(hanxia@wh.iov.cn) ; Yuan, zhiming(yzm@wh.iov.cn) |
| 英文摘要 | Objectives: banna virus (bav) is classified in the genus seadornavirus within the reoviridae family and considered to be an emerging pathogen. we aimed to develop a rapid and simple molecular detection approach for all bav subgroups in isothermal conditions. method: a set of six specific primers was designed to target the segment 12 of bav, and the reverse transcription-loop mediated isothermal amplification (rt-lamp) assay was developed and compared with conventional rt-pcr method. results: the amplification of the rt-lamp assay can be obtained within 40 min at 65 c. the results from specificity showed that only target bavs rna including genotypes a, b and c were amplified and the assay demonstrated a sensitivity of 3.6 10 2 pfu/ ml, which was higher than conventional rt-pcr measurement. a good reliability for the assay was presented in the further evaluation for bavs rna from serial diluted bav-spiked serum and 47 pools of field mosquito samples. conclusions: our findings present a rapid, sensitive and specific rt-lamp assay that can be applied for bav detection in clinical or field samples in the future. (c) 2018 the authors. published by elsevier ltd on behalf of international society for infectious diseases. |
| WOS关键词 | GENUS SEADORNAVIRUS ; COLTIVIRUSES |
| WOS研究方向 | Infectious Diseases |
| WOS类目 | Infectious Diseases |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000452810600015 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373210 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xia, Han; Yuan, Zhiming |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Henan Prov OriginBio Biotechnol Co LtD, Zhengzhou 450000, Henan, Peoples R China 4.Shanghai Cohere Elect Technol Co LtD, Shanghai 201101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Han,Zhao, Na,Zhao, Lu,et al. Rapid detection of banna virus by reverse transcription-loop -mediated isothermal amplification (rt-lamp)[J]. International journal of infectious diseases,2019,78:93-98. |
| APA | Xia, Han,Zhao, Na,Zhao, Lu,Wang, Yujuan,Zhao, Wang,&Yuan, Zhiming.(2019).Rapid detection of banna virus by reverse transcription-loop -mediated isothermal amplification (rt-lamp).International journal of infectious diseases,78,93-98. |
| MLA | Xia, Han,et al."Rapid detection of banna virus by reverse transcription-loop -mediated isothermal amplification (rt-lamp)".International journal of infectious diseases 78(2019):93-98. |
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