| Monoclonal antibody against ev71 3d(pol) inhibits the polymerase activity of rdrp and virus replication | |
| Li, Yaoming1; Yu, Jie1,2; Qi, Xiuwen1,2; Yan, Huimin1 | |
| 刊名 | Bmc immunology
 |
| 2019-01-22 | |
| 卷号 | 20页码:8 |
| 关键词 | Monoclonal antibody Enterovirus a 71 Rdrp Antiviral |
| ISSN号 | 1471-2237 |
| DOI | 10.1186/s12865-019-0288-x |
| 通讯作者 | Li, yaoming(liym@whsw.edu.cn) ; Yan, huimin(hmyan@wh.iov.cn) |
| 英文摘要 | Backgroundenterovirus a 71 (ev71) is a neurotropic virus that may lead to acute flaccid paralysis, encephalitis, cardiopulmonary failure or even death. no vaccine and defensive drug controlling ev71 is currently available, novel and efficient antiviral drug or vaccine is therefore urgently needed. 3d(pol) (rna-dependent rna polymerase (rdrp)) has been an important target for anti-ev71 drug development.methodsa panel of monoclonal igg antibodies (mabs) against ev71 3d(pol) were generated by traditional cell fusion methods. and the antibody affinity and specificity to ev71 3d(pol) were evaluated by enzyme-linked immunosorbent assay (elisa), indirect fluorescent assay (ifa) and western blotting. antiviral activities of these antibodies were also determined in vitro and in vivo.resultstwo mabs towards ev71 3d(pol) were able to effectively suppress ev71 replication in vero-1008 cell when intracellarly delivered. and they also dampened the rna polymerase activity of 3d(pol) in vitro. more importantly, these mabs provided partial protection in ev71-challenged neonatal murine challenge model.conclusionsthese results showed that two of mabs against ev71 3d(pol) inhibited ev71 replication and could be utilized as promising therapeutic drug candidate. |
| WOS关键词 | DEPENDENT RNA-POLYMERASE ; ENTEROVIRUS 71 ; MOUTH-DISEASE ; HAND ; FOOT ; NEUTRALIZATION ; INFECTION ; IMMUNITY ; MICE |
| WOS研究方向 | Immunology |
| WOS类目 | Immunology |
| 语种 | 英语 |
| 出版者 | BMC |
| WOS记录号 | WOS:000456397800001 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373200 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Li, Yaoming; Yan, Huimin |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Mucosal Immun Res Grp, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yaoming,Yu, Jie,Qi, Xiuwen,et al. Monoclonal antibody against ev71 3d(pol) inhibits the polymerase activity of rdrp and virus replication[J]. Bmc immunology,2019,20:8. |
| APA | Li, Yaoming,Yu, Jie,Qi, Xiuwen,&Yan, Huimin.(2019).Monoclonal antibody against ev71 3d(pol) inhibits the polymerase activity of rdrp and virus replication.Bmc immunology,20,8. |
| MLA | Li, Yaoming,et al."Monoclonal antibody against ev71 3d(pol) inhibits the polymerase activity of rdrp and virus replication".Bmc immunology 20(2019):8. |
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