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	Hiv-1 nuclear import in macrophages is regulated by cpsf6-capsid interactions at the nuclear pore complex
	Bejarano, David Alejandro1; Peng, Ke1,4; Laketa, Vibor1,2; Boerner, Kathleen1,2; Jost, K. Laurence1,2; Lucic, Bojana2,3; Glass, Baerbel1; Lusic, Marina2,3; Mueller, Barbara1; Kraeusslich, Hans-Georg1
刊名	Elife
	2019-01-23
卷号	8页码:31
ISSN号	2050-084X
DOI	10.7554/elife.41800
通讯作者	Peng, ke(pengke@wh.iov.cn) ; Kraeusslich, hans-georg(hans-georg.kraeusslich@med.uni-heidelberg.de)
英文摘要	Nuclear entry of hiv-1 replication complexes through intact nuclear pore complexes is critical for successful infection. the host protein cleavage-and-polyadenylation-specificity-factor-6 (cpsf6) has been implicated in different stages of early hiv-1 replication. applying quantitative microscopy of hiv-1 reverse-transcription and pre-integration-complexes (rtc/pic), we show that cpsf6 is strongly recruited to nuclear replication complexes but absent from cytoplasmic rtc/pic in primary human macrophages. depletion of cpsf6 or lack of cpsf6 binding led to accumulation of hiv-1 subviral complexes at the nuclear envelope of macrophages and reduced infectivity. two-color stimulated-emission-depletion microscopy indicated that under these circumstances hiv-1 complexes are retained inside the nuclear pore and undergo ca-multimer dependent cpsf6 clustering adjacent to the nuclear basket. we propose that nuclear entry of hiv-1 subviral complexes in macrophages is mediated by consecutive binding of nup153 and cpsf6 to the hexameric ca lattice.
WOS关键词	IMMUNODEFICIENCY-VIRUS TYPE-1 ; FACTOR-I-M ; HOST FACTORS ; CLEAVAGE ; INTEGRATION ; CPSF6 ; CELLS ; ENTRY ; RETROVIRUSES ; REPLICATION
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
语种	英语
出版者	ELIFE SCIENCES PUBLICATIONS LTD
WOS记录号	WOS:000460405100001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373185
专题	武汉病毒研究所
通讯作者	Peng, Ke; Kraeusslich, Hans-Georg
作者单位	1.Heidelberg Univ, Dept Infect Dis Virol, Heidelberg, Germany 2.German Ctr Infect Res, Heidelberg, Germany 3.Heidelberg Univ, Dept Infect Dis, Integrat Virol, Heidelberg, Germany 4.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Bejarano, David Alejandro,Peng, Ke,Laketa, Vibor,et al. Hiv-1 nuclear import in macrophages is regulated by cpsf6-capsid interactions at the nuclear pore complex[J]. Elife,2019,8:31.
APA	Bejarano, David Alejandro.,Peng, Ke.,Laketa, Vibor.,Boerner, Kathleen.,Jost, K. Laurence.,...&Kraeusslich, Hans-Georg.(2019).Hiv-1 nuclear import in macrophages is regulated by cpsf6-capsid interactions at the nuclear pore complex.Elife,8,31.
MLA	Bejarano, David Alejandro,et al."Hiv-1 nuclear import in macrophages is regulated by cpsf6-capsid interactions at the nuclear pore complex".Elife 8(2019):31.

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