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	A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for salmonella dna detection
	He, Enqi2,4; Cao, Ting1; Cai, Liangyuan1; Guo, Dan2; Zhou, Yinglin1; Zhang, Xinxiang1; Li, Zhihong3,4
刊名	Rsc advances
	2018
卷号	8期号:69页码:39561-39566
ISSN号	2046-2069
DOI	10.1039/c8ra07045h
通讯作者	Zhou, yinglin(zhouyl@pku.edu.cn) ; Zhang, xinxiang(zxx@pku.edu.cn) ; Li, zhihong(zhhli@pku.edu.cn)
英文摘要	In this work, a novel microcapsule array chip was fabricated for the detection of salmonella dna by integrating an ice-printing technique with dna isothermal amplification. reaction solutions were previously sealed in the microcapsule array chip via ice printing. to protect the relatively fragile dna isothermal amplification system, an extra polystyrene (ps) film was introduced to isolate the reaction solution from photopolymer precursor, which was proved to be a vital step for providing a clean and stable environment for dna amplification reaction. detection operation can be done by simply injecting sample dna into the microcapsule by an easily accessible syringe, and the result can be directly obtained through color change within 90 minutes. this method shows good sensitivity, specificity and stability.
WOS关键词	MICROFLUIDIC DEVICE ; POINT ; LAMP ; EXTRACTION ; BIOSENSOR ; ASSAY
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000452116300037
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372968
专题	大连化学物理研究所
通讯作者	Zhou, Yinglin; Zhang, Xinxiang; Li, Zhihong
作者单位	1.Peking Univ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, BNLMS,Minist Educ, Beijing 100871, Peoples R China 2.Tsinghua Univ, State Key Lab Tribol, Dept Mech Engn, Beijing 100084, Peoples R China 3.Peking Univ, Inst Microelect, Natl Key Lab Sci & Technol Micro Nano Fabricat, Beijing 100871, Peoples R China 4.Tsinghua Univ, Ctr Nano & Micro Mech, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	He, Enqi,Cao, Ting,Cai, Liangyuan,et al. A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for salmonella dna detection[J]. Rsc advances,2018,8(69):39561-39566.
APA	He, Enqi.,Cao, Ting.,Cai, Liangyuan.,Guo, Dan.,Zhou, Yinglin.,...&Li, Zhihong.(2018).A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for salmonella dna detection.Rsc advances,8(69),39561-39566.
MLA	He, Enqi,et al."A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for salmonella dna detection".Rsc advances 8.69(2018):39561-39566.

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