QQ客服 官方微博 反馈留言

	A novel two-dimensional liquid chromatography - mass spectrometry method for direct drug impurity identification from hplc eluent containing ion-pairing reagent in mobile phases
	Long, Zhen1; Zhan, Zhaoqi2; Guo, Zhimou3; Li, Yueqi1; Yao, Jinting1; Ji, Feng1; Li, Changkun1; Zheng, Xin1; Ren, Biao1; Huang, Taohong1
刊名	Analytica chimica acta
	2019-02-21
卷号	1049页码:105-114
关键词	Two dimensional liquid chromatography Ion-pairing reagent Non-volatile Mass spectrometry Generic drug Impurity
ISSN号	0003-2670
DOI	10.1016/j.aca.2018.10.031
通讯作者	Guo, zhimou(guozhimou@dicp.ac.cn) ; Li, yueqi(fxlyq@shimadzu.com.cn)
英文摘要	In this study, a novel two dimensional liquid chromatography - mass spectrometry (2d-lc-ms) method with use of a weak anion exchange column between the 1st dlc rp column and the 2nd dlc rp column (rp1-wax-rp2) was developed and applied to identify drug impurities from ms incompatible mobile phases containing sodium 1-octanesulfonate and non-volatile buffer. the 1st dlc conditions follow exactly the original standard hplc method recorded in chinese pharmacopeia (chp), european pharmacopeia (ep) or us pharmacopeia (usp). an impurity fraction was collected with a built-in sample loop (100 mu l) and transferred to the wax column where 1-octanesulfonate and phosphate were trapped and removed. while, the impurity and other cations were eluted to the 2nd d column (rp2) for separation and identification by connected it-tof ms. methods were programmed and applied to identify impurities in two generic drugs, sulpiride (hydrophilic drug with logp 0.57) and dobutamine (hydrophobic drug with logp 3.6). the results indicate that the methods based on rp1-wax-rp2 column configuration offer a feasible solution for direct impurity identification in generic drug product or api without needs of off-line desalting from the ms incompatible mobile phases containing ion-pairing reagent and nonvolatile buffer. (c) 2018 elsevier b.v. all rights reserved.
WOS关键词	BASIC COMPOUNDS ; SEPARATION ; ALKALOIDS ; COLUMN ; RETENTION
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000454789100012
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372927
专题	大连化学物理研究所
通讯作者	Guo, Zhimou; Li, Yueqi
作者单位	1.Shimadzu Sci Instrument Co, Beijing 100020, Peoples R China 2.Shimadzu Asia Pacific Pte Ltd, Singapore 118264, Singapore 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Long, Zhen,Zhan, Zhaoqi,Guo, Zhimou,et al. A novel two-dimensional liquid chromatography - mass spectrometry method for direct drug impurity identification from hplc eluent containing ion-pairing reagent in mobile phases[J]. Analytica chimica acta,2019,1049:105-114.
APA	Long, Zhen.,Zhan, Zhaoqi.,Guo, Zhimou.,Li, Yueqi.,Yao, Jinting.,...&Huang, Taohong.(2019).A novel two-dimensional liquid chromatography - mass spectrometry method for direct drug impurity identification from hplc eluent containing ion-pairing reagent in mobile phases.Analytica chimica acta,1049,105-114.
MLA	Long, Zhen,et al."A novel two-dimensional liquid chromatography - mass spectrometry method for direct drug impurity identification from hplc eluent containing ion-pairing reagent in mobile phases".Analytica chimica acta 1049(2019):105-114.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。