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	Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion cheek for fragmentation/neutral loss acquisition
	Li, Hua1; Qin, Qian2,4; Shi, Xianzhe2; He, Jun3; Xu, Guowang2
刊名	Journal of chromatography a
	2019-01-04
卷号	1583页码:80-87
关键词	Modified metabolites All ion fragmentation Neutral loss Nontargeted analysis Metabolomics
ISSN号	0021-9673
DOI	10.1016/j.chroma.2018.11.014
通讯作者	Shi, xianzhe(shixianzhe@dicp.ac.cn)
英文摘要	Modified metabolites play important roles in diagnostic monitoring, oxidative response and physiological regulation. comprehensive analytical methods are greatly needed for improving the coverage of modified metabolites and studying their physiological function. here, a novel nontargeted profiling method for mapping modified metabolites was developed by liquid chromatography-high resolution mass spectrometry with full scan/all ion fragmentation/neutral loss (fs/aif/nl) data acquisition. modified metabolites were unbiasedly defined with less false positive results by mixed standards verification. thirteen types of modified metabolites in urine were analyzed at the same time, and 198 of 307 modified metabolites at positive mode and 166 of 366 modified metabolites at negative mode were putatively identified. the modified metabolites profiling method was applied for investigating the metabolic differences between the patients with breast cancer and health controls. as a result, many modified metabolites with glu-curonidation, ribosylation and indole acetylation modification were significantly up-regulated in breast cancer and four modified metabolites including 7-methylguanosine, n-4-acetylcytidine, dihyroxy-1h-indole glucuronide i and indole-3-acetic acid-o-glucuronide were identified as potential biomarkers for the diagnose of breast cancer. (c) 2018 elsevier b.v. all rights reserved.
WOS关键词	MODIFICATION-SPECIFIC METABOLOMICS ; MODIFIED NUCLEOSIDES ; IDENTIFICATION ; PROTEOMICS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000455692100010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372864
专题	大连化学物理研究所
通讯作者	Shi, Xianzhe
作者单位	1.Southern Univ Sci & Technol, Mat Characterizat & Preparat Ctr, Shenzhen 518055, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Shekou Peoples Hosp, Dept clin lab, Shenzhen 518052, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Hua,Qin, Qian,Shi, Xianzhe,et al. Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion cheek for fragmentation/neutral loss acquisition[J]. Journal of chromatography a,2019,1583:80-87.
APA	Li, Hua,Qin, Qian,Shi, Xianzhe,He, Jun,&Xu, Guowang.(2019).Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion cheek for fragmentation/neutral loss acquisition.Journal of chromatography a,1583,80-87.
MLA	Li, Hua,et al."Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion cheek for fragmentation/neutral loss acquisition".Journal of chromatography a 1583(2019):80-87.

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